Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures.

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2022-06-10

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Abstract

Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expansion and maintenance of AT2s. The culture conditions are scalable and compatible with high-throughput chemical and genetic screenings and can potentially be used to generate large AT2 numbers for cell-based therapies. For complete details on the use and execution of this protocol, please refer to Katsura et al. (2020).

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Journal article

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Lung, Pulmonary Alveoli, Stem Cells, Animals, Humans, Mice, Cell Differentiation, Alveolar Epithelial Cells

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https://hdl.handle.net/10161/25525

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10.1016/j.xpro.2022.101447

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Konishi, Satoshi, Aleksandra Tata and Purushothama Rao Tata (2022). Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures. STAR protocols, 3(2). p. 101447. 10.1016/j.xpro.2022.101447 Retrieved from https://hdl.handle.net/10161/25525.

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Tata

Aleksandra Tata

Assistant Research Professor of Cell Biology
Tata

Purushothama Rao Tata

Associate Professor of Cell Biology

Lung regeneration
Lung stem cells
Cell plasticity
Organoid models
Lung Fibrosis
Single Cell Biology

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