Show simple item record

(15)N Hyperpolarization of Imidazole-(15)N2 for Magnetic Resonance pH Sensing via SABRE-SHEATH.

dc.contributor.author Shchepin, Roman V
dc.contributor.author Barskiy, Danila A
dc.contributor.author Coffey, Aaron M
dc.contributor.author Theis, Thomas
dc.contributor.author Shi, Fan
dc.contributor.author Warren, Warren S
dc.contributor.author Goodson, Boyd M
dc.contributor.author Chekmenev, Eduard Y
dc.coverage.spatial United States
dc.date.accessioned 2017-09-06T17:09:11Z
dc.date.available 2017-09-06T17:09:11Z
dc.date.issued 2016-06-24
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/27379344
dc.identifier.issn 2379-3694
dc.identifier.uri https://hdl.handle.net/10161/15446
dc.description.abstract (15)N nuclear spins of imidazole-(15)N2 were hyperpolarized using NMR signal amplification by reversible exchange in shield enables alignment transfer to heteronuclei (SABRE-SHEATH). A (15)N NMR signal enhancement of ∼2000-fold at 9.4 T is reported using parahydrogen gas (∼50% para-) and ∼0.1 M imidazole-(15)N2 in methanol:aqueous buffer (∼1:1). Proton binding to a (15)N site of imidazole occurs at physiological pH (pKa ∼ 7.0), and the binding event changes the (15)N isotropic chemical shift by ∼30 ppm. These properties are ideal for in vivo pH sensing. Additionally, imidazoles have low toxicity and are readily incorporated into a wide range of biomolecules. (15)N-Imidazole SABRE-SHEATH hyperpolarization potentially enables pH sensing on scales ranging from peptide and protein molecules to living organisms.
dc.language eng
dc.publisher American Chemical Society (ACS)
dc.relation.ispartof ACS Sens
dc.relation.isversionof 10.1021/acssensors.6b00231
dc.subject 15N
dc.subject NMR
dc.subject chemical shift
dc.subject hyperpolarization
dc.subject imidazole
dc.subject pH sensing
dc.subject parahydrogen
dc.title (15)N Hyperpolarization of Imidazole-(15)N2 for Magnetic Resonance pH Sensing via SABRE-SHEATH.
dc.type Journal article
duke.contributor.id Theis, Thomas|0598228
duke.contributor.id Warren, Warren S|0374058
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/27379344
pubs.begin-page 640
pubs.end-page 644
pubs.issue 6
pubs.organisational-group Chemistry
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Physics
pubs.organisational-group Radiology
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 1
duke.contributor.orcid Theis, Thomas|0000-0001-6779-9978


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record