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Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.

dc.contributor.author Petes, Thomas
dc.contributor.author Klein, Hannah L
dc.contributor.author Bačinskaja, Giedrė
dc.contributor.author Che, Jun
dc.contributor.author Cheblal, Anais
dc.contributor.author Elango, Rajula
dc.contributor.author Epshtein, Anastasiya
dc.contributor.author Fitzgerald, Devon M
dc.contributor.author Gómez-González, Belén
dc.contributor.author Khan, Sharik R
dc.contributor.author Kumar, Sandeep
dc.contributor.author Leland, Bryan A
dc.contributor.author Marie, Léa
dc.contributor.author Mei, Qian
dc.contributor.author Miné-Hattab, Judith
dc.contributor.author Piotrowska, Alicja
dc.contributor.author Polleys, Erica J
dc.contributor.author Putnam, Christopher D
dc.contributor.author Radchenko, Elina A
dc.contributor.author Saada, Anissia Ait
dc.contributor.author Sakofsky, Cynthia J
dc.contributor.author Shim, Eun Yong
dc.contributor.author Stracy, Mathew
dc.contributor.author Xia, Jun
dc.contributor.author Yan, Zhenxin
dc.contributor.author Yin, Yi
dc.contributor.author Aguilera, Andrés
dc.contributor.author Argueso, Juan Lucas
dc.contributor.author Freudenreich, Catherine H
dc.contributor.author Gasser, Susan M
dc.contributor.author Gordenin, Dmitry A
dc.contributor.author Haber, James E
dc.contributor.author Ira, Grzegorz
dc.contributor.author Jinks-Robertson, Sue
dc.contributor.author King, Megan C
dc.contributor.author Kolodner, Richard D
dc.contributor.author Kuzminov, Andrei
dc.contributor.author Lambert, Sarah Ae
dc.contributor.author Lee, Sang Eun
dc.contributor.author Miller, Kyle M
dc.contributor.author Mirkin, Sergei M
dc.contributor.author Rosenberg, Susan M
dc.contributor.author Rothstein, Rodney
dc.contributor.author Symington, Lorraine S
dc.contributor.author Zawadzki, Pawel
dc.contributor.author Kim, Nayun
dc.contributor.author Lisby, Michael
dc.contributor.author Malkova, Anna
dc.date.accessioned 2019-02-01T16:12:41Z
dc.date.available 2019-02-01T16:12:41Z
dc.date.issued 2019-01-07
dc.identifier MIC0178E138
dc.identifier.issn 2311-2638
dc.identifier.issn 2311-2638
dc.identifier.uri https://hdl.handle.net/10161/18039
dc.description.abstract Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
dc.language eng
dc.relation.ispartof Microbial cell (Graz, Austria)
dc.relation.isversionof 10.15698/mic2019.01.664
dc.subject DNA breaks
dc.subject DNA repair centers
dc.subject DNA resection
dc.subject DSBs
dc.subject Holliday junctions
dc.subject R-loops
dc.subject chromatin dynamics
dc.subject chromosome rearrangements
dc.subject crossovers
dc.subject fluorescent proteins
dc.subject gene amplification
dc.subject gene conversion
dc.subject genome instability
dc.subject gross chromosome rearrangements
dc.subject homologous recombination
dc.subject mitotic recombination
dc.subject mutagenesis
dc.subject pulsed field gel electrophoresis
dc.subject replication fork stalling
dc.subject single-particle tracking
dc.subject sister chromatid recombination
dc.subject sister repetitive sequences
dc.subject site-specific chromosome breaks
dc.subject toxic recombination intermediates
dc.subject yeast artificial chromosome
dc.title Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.
dc.type Journal article
dc.date.updated 2019-02-01T16:12:38Z
pubs.begin-page 1
pubs.end-page 64
pubs.issue 1
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Basic Science Departments
pubs.publication-status Published
pubs.volume 6


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