Show simple item record

Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.

dc.contributor.author Barkauskas, Christina
dc.contributor.author Hogan, Brigid
dc.contributor.author Bowie, Emily
dc.contributor.author Jain, Rajan
dc.contributor.author Takeda, Norifumi
dc.contributor.author Aghajanian, Haig
dc.contributor.author Wang, Qiaohong
dc.contributor.author Padmanabhan, Arun
dc.contributor.author Manderfield, Lauren J
dc.contributor.author Gupta, Mudit
dc.contributor.author Li, Deqiang
dc.contributor.author Li, Li
dc.contributor.author Trivedi, Chinmay M
dc.contributor.author Epstein, Jonathan A
dc.date.accessioned 2019-02-22T14:54:34Z
dc.date.available 2019-02-22T14:54:34Z
dc.date.issued 2015-04-13
dc.identifier ncomms7727
dc.identifier.issn 2041-1723
dc.identifier.issn 2041-1723
dc.identifier.uri https://hdl.handle.net/10161/18068
dc.description.abstract The plasticity of differentiated cells in adult tissues undergoing repair is an area of intense research. Pulmonary alveolar type II cells produce surfactant and function as progenitors in the adult, demonstrating both self-renewal and differentiation into gas exchanging type I cells. In vivo, type I cells are thought to be terminally differentiated and their ability to give rise to alternate lineages has not been reported. Here we show that Hopx becomes restricted to type I cells during development. However, unexpectedly, lineage-labelled Hopx(+) cells both proliferate and generate type II cells during adult alveolar regrowth following partial pneumonectomy. In clonal 3D culture, single Hopx(+) type I cells generate organoids composed of type I and type II cells, a process modulated by TGFβ signalling. These findings demonstrate unanticipated plasticity of type I cells and a bidirectional lineage relationship between distinct differentiated alveolar epithelial cell types in vivo and in single-cell culture.
dc.language eng
dc.publisher Springer Nature
dc.relation.ispartof Nature communications
dc.relation.isversionof 10.1038/ncomms7727
dc.subject Pulmonary Alveoli
dc.subject Clone Cells
dc.subject Epithelial Cells
dc.subject Animals
dc.subject Mice, Transgenic
dc.subject Humans
dc.subject Mice
dc.subject Tamoxifen
dc.subject Transforming Growth Factor beta
dc.subject Homeodomain Proteins
dc.subject Green Fluorescent Proteins
dc.subject Pneumonectomy
dc.subject Cell Culture Techniques
dc.subject Crosses, Genetic
dc.subject Regeneration
dc.subject Signal Transduction
dc.subject Cell Differentiation
dc.subject Cell Proliferation
dc.subject Gene Expression
dc.subject Cell Lineage
dc.subject Genes, Reporter
dc.subject Male
dc.subject Cell Tracking
dc.title Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung.
dc.type Journal article
dc.date.updated 2019-02-22T14:54:30Z
pubs.begin-page 6727
pubs.issue 1
pubs.organisational-group School of Medicine
pubs.organisational-group Duke
pubs.organisational-group Medicine, Pulmonary, Allergy, and Critical Care Medicine
pubs.organisational-group Medicine
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Cell Biology
pubs.organisational-group Basic Science Departments
pubs.organisational-group Pediatrics
pubs.organisational-group Student
pubs.publication-status Published
pubs.volume 6


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record