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Potentiation of beta-adrenergic signaling by adenoviral-mediated gene transfer in adult rabbit ventricular myocytes.

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Date
1997-01-15
Authors
Drazner, MH
Peppel, KC
Dyer, S
Grant, AO
Koch, WJ
Lefkowitz, RJ
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Abstract
Our laboratory has been testing the hypothesis that genetic modulation of the beta-adrenergic signaling cascade can enhance cardiac function. We have previously shown that transgenic mice with cardiac overexpression of either the human beta2-adrenergic receptor (beta2AR) or an inhibitor of the beta-adrenergic receptor kinase (betaARK), an enzyme that phosphorylates and uncouples agonist-bound receptors, have increased myocardial inotropy. We now have created recombinant adenoviruses encoding either the beta2AR (Adeno-beta2AR) or a peptide betaARK inhibitor (consisting of the carboxyl terminus of betaARK1, Adeno-betaARKct) and tested their ability to potentiate beta-adrenergic signaling in cultured adult rabbit ventricular myocytes. As assessed by radioligand binding, Adeno-beta2AR infection led to approximately 20-fold overexpression of beta-adrenergic receptors. Protein immunoblots demonstrated the presence of the Adeno-betaARKct transgene. Both transgenes significantly increased isoproterenol-stimulated cAMP as compared to myocytes infected with an adenovirus encoding beta-galactosidase (Adeno-betaGal) but did not affect the sarcolemmal adenylyl cyclase response to Forskolin or NaF. beta-Adrenergic agonist-induced desensitization was significantly inhibited in Adeno-betaARKct-infected myocytes (16+/-2%) as compared to Adeno-betaGal-infected myocytes (37+/-1%, P < 0.001). We conclude that recombinant adenoviral gene transfer of the beta2AR or an inhibitor of betaARK-mediated desensitization can potentiate beta-adrenergic signaling.
Type
Journal article
Subject
Adenoviridae
Adenylyl Cyclases
Adrenergic beta-Agonists
Animals
Cell Survival
Cells, Cultured
Cyclic AMP
Gene Transfer Techniques
Genetic Vectors
Heart Ventricles
Humans
Isoproterenol
Male
Rabbits
Receptors, Adrenergic, beta-2
Sarcolemma
Signal Transduction
Transgenes
Permalink
https://hdl.handle.net/10161/7831
Published Version (Please cite this version)
10.1172/JCI119157
Publication Info
Drazner, MH; Peppel, KC; Dyer, S; Grant, AO; Koch, WJ; & Lefkowitz, RJ (1997). Potentiation of beta-adrenergic signaling by adenoviral-mediated gene transfer in adult rabbit ventricular myocytes. J Clin Invest, 99(2). pp. 288-296. 10.1172/JCI119157. Retrieved from https://hdl.handle.net/10161/7831.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Grant

Augustus Oliver Grant

Professor of Medicine
This laboratory seeks to understand the molecular events that control excitation in cardiac muscle and the mechanisms by which excitation is modified by drugs. The experimental models include the isolated cardiac myocyte, the frog oocyte and mammalian cells expressing mRNA transcripts of wild-type and mutant channels. The experimental techniques include DNA and RNA manipulation, whole cell voltage clamping and the extracellular patch clamping. The current focus of the laboratory i
Lefkowitz

Robert J. Lefkowitz

The Chancellor's Distinguished Professor of Medicine
Dr. Lefkowitz’s memoir, A Funny Thing Happened on the Way to Stockholm, recounts his early career as a cardiologist and his transition to biochemistry, which led to his Nobel Prize win. Robert J. Lefkowitz, M.D. is James B. Duke Professor of Medicine and Professor of Biochemistry and Chemistry at the Duke University Medical Center. He has been an Investigator of the
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