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Molecular Mechanisms of Notochord Vacuole Formation and Their Role in Zebrafish Development

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Date
2014
Author
Ellis, Kathryn Leigh
Advisor
Bagnat, Michel
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Abstract

The notochord plays critical structural and signaling roles during vertebrate development. At the center of the vertebrate notochord is a large fluid-filled organelle, the notochord vacuole. While these highly conserved intracellular structures have been described for decades, little is known about the molecular mechanisms involved in their biogenesis and maintenance. Here we show that zebrafish notochord vacuoles are specialized lysosome-related organelles whose formation and maintenance requires late endosomal trafficking regulated by the vacuole-specific Rab32a, and H+-ATPase-dependent acidification. We establish that notochord vacuoles are required for body axis elongation during embryonic development and identify a novel role for notochord vacuoles in spine morphogenesis. Thus, the vertebrate notochord plays important structural roles beyond early development.

Type
Dissertation
Department
Cell Biology
Subject
Cellular biology
Developmental biology
lysosome-related organelle
notochord
scoliosis
vacuole
vertebrae
zebrafish
Permalink
https://hdl.handle.net/10161/8723
Citation
Ellis, Kathryn Leigh (2014). Molecular Mechanisms of Notochord Vacuole Formation and Their Role in Zebrafish Development. Dissertation, Duke University. Retrieved from https://hdl.handle.net/10161/8723.
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