Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.
| dc.contributor.author | Regan, JW | |
| dc.contributor.author | Kobilka, TS | |
| dc.contributor.author | Yang-Feng, TL | |
| dc.contributor.author | Caron, MG | |
| dc.contributor.author | Lefkowitz, RJ | |
| dc.contributor.author | Kobilka, BK | |
| dc.coverage.spatial | United States | |
| dc.date.accessioned | 2013-09-24T17:45:42Z | |
| dc.date.issued | 1988-09 | |
| dc.description.abstract | An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic ligands. | |
| dc.identifier | ||
| dc.identifier.issn | 0027-8424 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Proceedings of the National Academy of Sciences | |
| dc.relation.ispartof | Proc Natl Acad Sci U S A | |
| dc.subject | Amino Acid Sequence | |
| dc.subject | Animals | |
| dc.subject | Base Sequence | |
| dc.subject | Cell Line | |
| dc.subject | Chromosomes, Human, Pair 4 | |
| dc.subject | Cloning, Molecular | |
| dc.subject | DNA | |
| dc.subject | Genes | |
| dc.subject | Humans | |
| dc.subject | Kidney | |
| dc.subject | Kinetics | |
| dc.subject | Models, Molecular | |
| dc.subject | Molecular Sequence Data | |
| dc.subject | Protein Conformation | |
| dc.subject | Receptors, Adrenergic, alpha | |
| dc.subject | Transcription, Genetic | |
| dc.title | Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Lefkowitz, RJ|0000-0003-1472-7545 | |
| pubs.author-url | ||
| pubs.begin-page | 6301 | |
| pubs.end-page | 6305 | |
| pubs.issue | 17 | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Biochemistry | |
| pubs.organisational-group | Cell Biology | |
| pubs.organisational-group | Chemistry | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Duke Institute for Brain Sciences | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | Medicine | |
| pubs.organisational-group | Medicine, Cardiology | |
| pubs.organisational-group | Neurobiology | |
| pubs.organisational-group | Pathology | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Trinity College of Arts & Sciences | |
| pubs.organisational-group | University Institutes and Centers | |
| pubs.publication-status | Published | |
| pubs.volume | 85 |
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