Adaptive Optics in Multiphoton Microscopy

dc.contributor.advisor

Warren, Warren S

dc.contributor.author

Kemeldinov, Aidyn

dc.date.accessioned

2017-08-16T18:26:11Z

dc.date.available

2017-08-16T18:26:11Z

dc.date.issued

2017

dc.department

Physics

dc.description.abstract

Any type of microscopy faces the problem in an attenuated signal level and reduced optical resolution due to optical aberration. To overcome this problem, adaptive optics was implemented in a two-photon fluorescence microscope. Using a “sensorless” approach, this study corrected the aberration in the system using two different excitation colors. As methodology, the point spread function was compared before and after applying adaptive optics. This study demonstrated that adaptive optics improves the resolution of the microscope for both excitation wavelengths. The aberration correction was then monitored as a function of depth. The result showed an improvement in the optimization metric as imaging depth is increased. Thus, adaptive optics offers improved imaging of the sample at deeper depths with better optical resolution and higher signal-to-noise ratio.

dc.identifier.uri

https://hdl.handle.net/10161/15262

dc.subject

Optics

dc.subject

Physics

dc.subject

Adaptive optics

dc.subject

Multiphoton microscopy

dc.title

Adaptive Optics in Multiphoton Microscopy

dc.type

Master's thesis

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Kemeldinov_duke_0066N_14016.pdf
Size:
1.58 MB
Format:
Adobe Portable Document Format

Collections