Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus.

Liu, Jie

Ochieng, Caroline

Wiersma, Steve

Ströher, Ute

Towner, Jonathan S

Whitmer, Shannon

Nichol, Stuart T

Moore, Christopher C

Kersh, Gilbert J

Kato, Cecilia

Sexton, Christopher

Petersen, Jeannine

Massung, Robert

Hercik, Christine

Crump, John A

Kibiki, Gibson

Maro, Athanasia

Mujaga, Buliga

Gratz, Jean

Jacob, Shevin T

Banura, Patrick

Scheld, W Michael

Juma, Bonventure

Onyango, Clayton O

Montgomery, Joel M

Houpt, Eric

Fields, Barry

McAdam, AJ

United States

2017-03-02T19:00:25Z

2017-03-02T19:00:25Z

2016-01

Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (Bartonella spp., Brucella spp., Coxiella burnetii, Leptospira spp., Rickettsia spp., Salmonella enterica and Salmonella enterica serovar Typhi, and Yersinia pestis), and 3 protozoa (Leishmania spp., Plasmodium spp., and Trypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.

https://www.ncbi.nlm.nih.gov/pubmed/26491176

JCM.02257-15

1098-660X

https://hdl.handle.net/10161/13764

eng

American Society for Microbiology

J Clin Microbiol

10.1128/JCM.02257-15

Adult

Communicable Diseases

Disease Outbreaks

Epidemiological Monitoring

Fever of Unknown Origin

Humans

Molecular Diagnostic Techniques

Real-Time Polymerase Chain Reaction

Reference Standards

Sensitivity and Specificity

Time Factors

Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus.

Journal article

https://www.ncbi.nlm.nih.gov/pubmed/26491176

49

58

1

Clinical Science Departments

Duke

Medicine

Medicine, Infectious Diseases

Pathology

School of Medicine

Published

54