Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays

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Staats, Janet S

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Enzor, Jennifer H

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Sanchez, Ana M

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Rountree, Wes

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Chan, Cliburn

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Jaimes, Maria

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Chan, Ray Chun-Fai

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Gaur, Amitabh

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Denny, Thomas N

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Weinhold, Kent J

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2017-06-01T19:41:03Z

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2017-06-01T19:41:03Z

dc.date.issued

2014-01-01

dc.description.abstract

The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular Cytokine Staining (ICS) assays. The goal of the EQAPOL Flow Cytometry External Quality Assurance Program (EQAP) is to provide proficiency testing and remediation for participating sites. The program is not punitive; rather, EQAPOL aims to help sites identify areas for improvement. EQAPOL utilizes a highly standardized ICS assay to minimize variability and readily identify those sites experiencing technical difficulties with their assays. Here, we report the results of External Proficiency 3 (EP3) where participating sites performed a 7-color ICS assay. On average, sites perform well in the Flow Cytometry EQAP (median score is "Good"). The most common technical issues identified by the program involve protocol adherence and data analysis; these areas have been the focus of site remediation. The EQAPOL Flow Cytometry team is now in the process of expanding the program to 8-color ICS assays. Evaluating polyfunctional ICS responses would align the program with assays currently being performed in support of HIV immune monitoring assays. © 2014 Elsevier B.V.

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1872-7905

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0022-1759

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https://hdl.handle.net/10161/14694

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Elsevier BV

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Journal of Immunological Methods

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10.1016/j.jim.2014.05.021

dc.title

Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays

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Other article

duke.contributor.orcid

Chan, Cliburn|0000-0001-5901-6806

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44

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53

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Basic Science Departments

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Biostatistics & Bioinformatics

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Clinical Science Departments

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Duke

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Duke Cancer Institute

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Duke Human Vaccine Institute

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Immunology

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Institutes and Centers

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Medicine

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Medicine, Duke Human Vaccine Institute

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Pathology

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School of Medicine

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Statistical Science

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Surgery

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Surgery, Surgical Sciences

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Trinity College of Arts & Sciences

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Published

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409

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