Cell surface glycoproteomic analysis of prostate cancer-derived PC-3 cells.

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2011-09

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Abstract

Most clinically approved biomarkers of cancer are glycoproteins, and those residing on the cell surface are of particular interest in biotherapeutics. We report a method for selective labeling, affinity enrichment, and identification of cell-surface glycoproteins. PC-3 cells and primary human prostate cancer tissue were treated with peracetylated N-azidoacetylgalactosamine, resulting in metabolic labeling of cell surface glycans with the azidosugar. We used mass spectrometry to identify over 70 cell surface glycoproteins and biochemically validated CD146 and integrin beta-4, both of which are known to promote metastatic behavior. These results establish cell-surface glycoproteomics as an effective technique for discovery of cancer biomarkers.

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10.1016/j.bmcl.2011.05.045

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Hubbard, Sarah C, Michael Boyce, Cheryl T McVaugh, Donna M Peehl and Carolyn R Bertozzi (2011). Cell surface glycoproteomic analysis of prostate cancer-derived PC-3 cells. Bioorganic & medicinal chemistry letters, 21(17). pp. 4945–4950. 10.1016/j.bmcl.2011.05.045 Retrieved from https://hdl.handle.net/10161/19699.

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Boyce

Michael Scott Boyce

Associate Professor of Biochemistry

The Boyce Lab studies mammalian cell signaling through protein glycosylation. For the latest news, project information and publications from our group, please visit our web site at http://www.boycelab.org or follow us on Twitter at https://twitter.com/BoyceLab.


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