Search for microRNAs expressed by intracellular bacterial pathogens in infected mammalian cells.

dc.contributor.author

Furuse, Yuki

dc.contributor.author

Finethy, Ryan

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Saka, Hector A

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Xet-Mull, Ana M

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Sisk, Dana M

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Smith, Kristen L Jurcic

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Lee, Sunhee

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Coers, Jörn

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Valdivia, Raphael H

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Tobin, David M

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Cullen, Bryan R

dc.contributor.editor

Rudel, Thomas

dc.coverage.spatial

United States

dc.date.accessioned

2015-12-16T23:03:10Z

dc.date.issued

2014

dc.description.abstract

MicroRNAs are expressed by all multicellular organisms and play a critical role as post-transcriptional regulators of gene expression. Moreover, different microRNA species are known to influence the progression of a range of different diseases, including cancer and microbial infections. A number of different human viruses also encode microRNAs that can attenuate cellular innate immune responses and promote viral replication, and a fungal pathogen that infects plants has recently been shown to express microRNAs in infected cells that repress host cell immune responses and promote fungal pathogenesis. Here, we have used deep sequencing of total expressed small RNAs, as well as small RNAs associated with the cellular RNA-induced silencing complex RISC, to search for microRNAs that are potentially expressed by intracellular bacterial pathogens and translocated into infected animal cells. In the case of Legionella and Chlamydia and the two mycobacterial species M. smegmatis and M. tuberculosis, we failed to detect any bacterial small RNAs that had the characteristics expected for authentic microRNAs, although large numbers of small RNAs of bacterial origin could be recovered. However, a third mycobacterial species, M. marinum, did express an ∼ 23-nt small RNA that was bound by RISC and derived from an RNA stem-loop with the characteristics expected for a pre-microRNA. While intracellular expression of this candidate bacterial microRNA was too low to effectively repress target mRNA species in infected cultured cells in vitro, artificial overexpression of this potential bacterial pre-microRNA did result in the efficient repression of a target mRNA. This bacterial small RNA therefore represents the first candidate microRNA of bacterial origin.

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/25184567

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PONE-D-14-24223

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1932-6203

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https://hdl.handle.net/10161/11186

dc.language

eng

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Public Library of Science (PLoS)

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PLoS One

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10.1371/journal.pone.0106434

dc.subject

Carboxypeptidases

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Chlamydia

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Gene Expression Regulation

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High-Throughput Nucleotide Sequencing

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Host-Pathogen Interactions

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Humans

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Immunity, Innate

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Legionella

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MicroRNAs

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Mycobacterium tuberculosis

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RNA, Bacterial

dc.title

Search for microRNAs expressed by intracellular bacterial pathogens in infected mammalian cells.

dc.type

Journal article

duke.contributor.orcid

Coers, Jörn|0000-0001-8707-4608

duke.contributor.orcid

Valdivia, Raphael H|0000-0003-0961-073X

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Tobin, David M|0000-0003-3465-5518

duke.contributor.orcid

Cullen, Bryan R|0000-0002-8638-6850

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/25184567

pubs.begin-page

e106434

pubs.issue

9

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Basic Science Departments

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Clinical Science Departments

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Duke

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Duke Cancer Institute

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Duke Human Vaccine Institute

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Immunology

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Institutes and Centers

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Medicine

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Medicine, Rheumatology and Immunology

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Molecular Genetics and Microbiology

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Pathology

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School of Medicine

pubs.publication-status

Published online

pubs.volume

9

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