Quantification of the humidity effect on HR by Ion leakage assay.

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2019-04-05

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Abstract

We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the hypersensitive response (HR), the ETI-associated cell death. The method is simple and short enough to handle many biological replicates, which improves the power of test of statistical significance. The protocol is easily applicable to other environmental cues, such as light and temperature, or treatment with chemicals.

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10.21769/bioprotoc.3203

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Mwimba, Musoki, and Xinnian Dong (2019). Quantification of the humidity effect on HR by Ion leakage assay. Bio-protocol, 9(7). p. e3203. 10.21769/bioprotoc.3203 Retrieved from https://hdl.handle.net/10161/22487.

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Dong

Xinnian Dong

Arts and Sciences Distinguished Professor of Biology

Using Arabidopsis thaliana as a model system, my laboratory studies the mechanisms of plant defense against microbial pathogens. We focus on a specific response known as systemic acquired resistance (SAR). SAR, which can be induced by a local infection, provides the plants with long lasting, systemic resistance against a broad spectrum of pathogens. Salicylic acid (SA; an active ingredient of aspirin) has been found to be the endogenous signal of SAR. Using a genetic approach, our laboratory identified genes involved in the regulation of SAR. Molecular and genetic analyses are being carried out to understand the gene function and to elucidate the SAR signaling pathway. These SAR-regulating genes are also favorite targets for molecular engineering of disease-resistance crops.


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