Formation of retinal direction-selective circuitry initiated by starburst amacrine cell homotypic contact.
| dc.contributor.author | Ray, Thomas A | |
| dc.contributor.author | Roy, Suva | |
| dc.contributor.author | Kozlowski, Christopher | |
| dc.contributor.author | Wang, Jingjing | |
| dc.contributor.author | Cafaro, Jon | |
| dc.contributor.author | Hulbert, Samuel W | |
| dc.contributor.author | Wright, Christopher V | |
| dc.contributor.author | Field, Greg D | |
| dc.contributor.author | Kay, Jeremy N | |
| dc.date.accessioned | 2018-05-01T13:26:36Z | |
| dc.date.available | 2018-05-01T13:26:36Z | |
| dc.date.issued | 2018-04-03 | |
| dc.date.updated | 2018-05-01T13:26:33Z | |
| dc.description.abstract | A common strategy by which developing neurons locate their synaptic partners is through projections to circuit-specific neuropil sublayers. Once established, sublayers serve as a substrate for selective synapse formation, but how sublayers arise during neurodevelopment remains unknown. Here we identify the earliest events that initiate formation of the direction-selective circuit in the inner plexiform layer of mouse retina. We demonstrate that radially-migrating newborn starburst amacrine cells establish homotypic contacts on arrival at the inner retina. These contacts, mediated by the cell-surface protein MEGF10, trigger neuropil innervation resulting in generation of two sublayers comprising starburst-cell dendrites. This dendritic scaffold then recruits projections from circuit partners. Abolishing MEGF10-mediated contacts profoundly delays and ultimately disrupts sublayer formation, leading to broader direction tuning and weaker direction-selectivity in retinal ganglion cells. Our findings reveal a mechanism by which differentiating neurons transition from migratory to mature morphology, and highlight this mechanism's importance in forming circuit-specific sublayers. | |
| dc.identifier.issn | 2050-084X | |
| dc.identifier.issn | 2050-084X | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | eLife Sciences Publications, Ltd | |
| dc.relation.ispartof | eLife | |
| dc.relation.isversionof | 10.7554/elife.34241 | |
| dc.subject | mouse | |
| dc.subject | neuroscience | |
| dc.title | Formation of retinal direction-selective circuitry initiated by starburst amacrine cell homotypic contact. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Hulbert, Samuel W|0000-0003-0369-0150 | |
| duke.contributor.orcid | Field, Greg D|0000-0001-5942-2679 | |
| duke.contributor.orcid | Kay, Jeremy N|0000-0001-6145-1604 | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Neurobiology | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Duke Science & Society | |
| pubs.organisational-group | Initiatives | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | Ophthalmology | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Biomedical Engineering | |
| pubs.organisational-group | Pratt School of Engineering | |
| pubs.organisational-group | Duke Institute for Brain Sciences | |
| pubs.organisational-group | University Institutes and Centers | |
| pubs.publication-status | Published | |
| pubs.volume | 7 |