Analysis of the Interaction between Viruses, Mirnas and the Rnai Pathway

Loading...
Thumbnail Image

Date

2008-04-03

Journal Title

Journal ISSN

Volume Title

Repository Usage Stats

449
views
897
downloads

Abstract

The microRNA (miRNA) and RNA interference (RNAi) pathways have recently emerged as an important aspect of virus-host cell interaction. This interaction can occur in several different ways and may favor either the virus or the host cell. Plants and invertebrates use RNAi as a first line of defense against virus infection by cleaving long, double-stranded viral transcripts into small interfering RNAs. However, it remains to be determined whether mammalian cells also initiate a similar response to infection. Here we present evidence that mammalian cells in fact do not induce an antiviral RNAi defense in response to infection by primate retroviruses.

Viruses may also interact with host cells by encoding miRNAs to regulate either cellular or viral gene expression. Here we demonstrate that herpes simplex virus type 1 (HSV-1) encodes at least five miRNAs which are primarily expressed during latency. Two of these miRNAs modulate expression of viral genes required for productive replication. We hypothesize that down regulation of these viral genes by these latency associated miRNAs allows HSV-1 to establish and maintain the latent state.

Description

Provenance

Citation

Citation

Umbach, Jennifer Lin (2008). Analysis of the Interaction between Viruses, Mirnas and the Rnai Pathway. Dissertation, Duke University. Retrieved from https://hdl.handle.net/10161/596.

Collections


Dukes student scholarship is made available to the public using a Creative Commons Attribution / Non-commercial / No derivative (CC-BY-NC-ND) license.