(15)N Hyperpolarization of Imidazole-(15)N2 for Magnetic Resonance pH Sensing via SABRE-SHEATH.

Shchepin, Roman V

Barskiy, Danila A

Coffey, Aaron M

Theis, Thomas

Shi, Fan

Warren, Warren S

Goodson, Boyd M

Chekmenev, Eduard Y

United States

2017-09-06T17:09:11Z

2017-09-06T17:09:11Z

2016-06-24

(15)N nuclear spins of imidazole-(15)N2 were hyperpolarized using NMR signal amplification by reversible exchange in shield enables alignment transfer to heteronuclei (SABRE-SHEATH). A (15)N NMR signal enhancement of ∼2000-fold at 9.4 T is reported using parahydrogen gas (∼50% para-) and ∼0.1 M imidazole-(15)N2 in methanol:aqueous buffer (∼1:1). Proton binding to a (15)N site of imidazole occurs at physiological pH (pKa ∼ 7.0), and the binding event changes the (15)N isotropic chemical shift by ∼30 ppm. These properties are ideal for in vivo pH sensing. Additionally, imidazoles have low toxicity and are readily incorporated into a wide range of biomolecules. (15)N-Imidazole SABRE-SHEATH hyperpolarization potentially enables pH sensing on scales ranging from peptide and protein molecules to living organisms.

https://www.ncbi.nlm.nih.gov/pubmed/27379344

2379-3694

https://hdl.handle.net/10161/15446

eng

American Chemical Society (ACS)

ACS Sens

10.1021/acssensors.6b00231

15N

NMR

chemical shift

hyperpolarization

imidazole

pH sensing

parahydrogen

(15)N Hyperpolarization of Imidazole-(15)N2 for Magnetic Resonance pH Sensing via SABRE-SHEATH.

Journal article

Theis, Thomas|0000-0001-6779-9978

https://www.ncbi.nlm.nih.gov/pubmed/27379344

640

644

6

Chemistry

Clinical Science Departments

Duke

Duke Cancer Institute

Institutes and Centers

Physics

Radiology

School of Medicine

Trinity College of Arts & Sciences

Published

1