The Contribution of Horizontal Gene Transfer to the Evolution of Fungi.

dc.contributor.advisor

Dietrich, Fred S

dc.contributor.advisor

Heitman, Joseph

dc.contributor.advisor

Lutzoni, Francois

dc.contributor.advisor

McCusker, John

dc.contributor.advisor

Mitchell, Thomas

dc.contributor.advisor

Rausher, Mark

dc.contributor.author

Hall, Charles Robert

dc.date.accessioned

2007-05-10T14:55:20Z

dc.date.available

2007-05-10T14:55:20Z

dc.date.issued

2007-05-10T14:55:20Z

dc.department

Molecular Genetics and Microbiology

dc.description.abstract

The genomes of the hemiascomycetes Saccharomyces cerevisiae and Ashbya gossypii have been completely sequenced, allowing a comparative analysis of these two genomes, which reveals that a small number of genes appear to have entered these genomes as a result of horizontal gene transfer from bacterial sources. One potential case of horizontal gene transfer in A. gossypii and 10 potential cases in S. cerevisiae were identified, of which two were investigated further. One gene, encoding the enzyme dihydroorotate dehydrogenase (DHOD), is potentially a case of horizontal gene transfer, as shown by sequencing of this gene from additional bacterial and fungal species to generate sufficient data to construct a well-supported phylogeny. The DHOD-encoding gene found in S. cerevisiae, URA1 (YKL216W), appears to have entered the Saccharomycetaceae after the divergence of the S. cerevisiae lineage from the Candida albicans lineage and possibly since the divergence from the A. gossypii lineage. This gene appears to have come from the Lactobacillales, and following its acquisition the endogenous eukaryotic DHOD gene was lost. It was also shown that the bacterially derived horizontally transferred DHOD is required for anaerobic synthesis of uracil in S. cerevisiae. The other gene discussed in detail is BDS1, an aryl- and alkyl-sulfatase gene of bacterial origin that we have shown allows utilization of sulfate from several organic sources. Among the eukaryotes, this gene is found in S. cerevisiae and Saccharomyces bayanus and appears to derive from the alpha-proteobacteria.

dc.format.extent

15275878 bytes

dc.format.mimetype

application/pdf

dc.identifier.uri

https://hdl.handle.net/10161/202

dc.language.iso

en_US

dc.subject

horizontal gene transfer

dc.subject

lateral gene transfer

dc.subject

Saccharomyces cerevisiae

dc.subject

biotin

dc.subject

uracil

dc.subject

sulfatase

dc.title

The Contribution of Horizontal Gene Transfer to the Evolution of Fungi.

dc.type

Dissertation

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
D_Hall_Charles_Robert_a_052007.pdf
Size:
14.57 MB
Format:
Adobe Portable Document Format

Collections