External Quality Assessment Program for Next-Generation Sequencing-Based HIV Drug Resistance Testing: Logistical Considerations.

dc.contributor.author

Ji, Hezhao

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Parkin, Neil

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Gao, Feng

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Denny, Thomas

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Jennings, Cheryl

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Sandstrom, Paul

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Kantor, Rami

dc.date.accessioned

2021-01-04T20:59:59Z

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2021-01-04T20:59:59Z

dc.date.issued

2020-05-18

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2021-01-04T20:59:58Z

dc.description.abstract

Next-generation sequencing (NGS) is likely to become the new standard method for HIV drug resistance (HIVDR) genotyping. Despite the significant advances in the development of wet-lab protocols and bioinformatic data processing pipelines, one often-missing critical component of an NGS HIVDR assay for clinical use is external quality assessment (EQA). EQA is essential for ensuring assay consistency and laboratory competency in performing routine biomedical assays, and the rollout of NGS HIVDR tests in clinical practice will require an EQA. In September 2019, the 2nd International Symposium on NGS HIVDR was held in Winnipeg, Canada. It convened a multidisciplinary panel of experts, including research scientists, clinicians, bioinformaticians, laboratory biologists, biostatisticians, and EQA experts. A themed discussion was conducted on EQA strategies towards such assays during the symposium. This article describes the logistical challenges identified and summarizes the opinions and recommendations derived from these discussions, which may inform the development of an inaugural EQA program for NGS HIVDR in the near future.

dc.identifier

v12050556

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1999-4915

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1999-4915

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https://hdl.handle.net/10161/21995

dc.language

eng

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MDPI AG

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Viruses

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10.3390/v12050556

dc.subject

HIV

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drug resistance

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external quality assessment

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next generation sequencing

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External Quality Assessment Program for Next-Generation Sequencing-Based HIV Drug Resistance Testing: Logistical Considerations.

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Journal article

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Gao, Feng|0000-0001-8903-0203

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556

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556

pubs.issue

5

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School of Medicine

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Duke Human Vaccine Institute

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Duke Global Health Institute

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Medicine, Duke Human Vaccine Institute

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Duke

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Institutes and Centers

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University Institutes and Centers

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Institutes and Provost's Academic Units

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Medicine

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Clinical Science Departments

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Medicine, Infectious Diseases

pubs.publication-status

Published

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12

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