Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated.

Liao, Hua-Xin

Chen, Xi

Munshaw, Supriya

Zhang, Ruijun

Marshall, Dawn J

Vandergrift, Nathan

Whitesides, John F

Lu, Xiaozhi

Yu, Jae-Sung

Hwang, Kwan-Ki

Gao, Feng

Markowitz, Martin

Heath, Sonya L

Bar, Katharine J

Goepfert, Paul A

Montefiori, David C

Shaw, George C

Alam, S Munir

Margolis, David M

Denny, Thomas N

Boyd, Scott D

Marshal, Eleanor

Egholm, Michael

Simen, Birgitte B

Hanczaruk, Bozena

Fire, Andrew Z

Voss, Gerald

Kelsoe, Garnett

Tomaras, Georgia D

Moody, M Anthony

Kepler, Thomas B

Haynes, Barton F

United States

2017-06-02T12:36:48Z

2017-06-02T12:36:48Z

2011-10-24

The initial antibody response to HIV-1 is targeted to envelope (Env) gp41, and is nonneutralizing and ineffective in controlling viremia. To understand the origins and characteristics of gp41-binding antibodies produced shortly after HIV-1 transmission, we isolated and studied gp41-reactive plasma cells from subjects acutely infected with HIV-1. The frequencies of somatic mutations were relatively high in these gp41-reactive antibodies. Reverted unmutated ancestors of gp41-reactive antibodies derived from subjects acutely infected with HIV-1 frequently did not react with autologous HIV-1 Env; however, these antibodies were polyreactive and frequently bound to host or bacterial antigens. In one large clonal lineage of gp41-reactive antibodies, reactivity to HIV-1 Env was acquired only after somatic mutations. Polyreactive gp41-binding antibodies were also isolated from uninfected individuals. These data suggest that the majority of gp41-binding antibodies produced after acute HIV-1 infection are cross-reactive responses generated by stimulating memory B cells that have previously been activated by non-HIV-1 antigens.

https://www.ncbi.nlm.nih.gov/pubmed/21987658

jem.20110363

1540-9538

https://hdl.handle.net/10161/14733

eng

Rockefeller University Press

J Exp Med

10.1084/jem.20110363

Adult

Cell Lineage

Female

HIV Antibodies

HIV Envelope Protein gp41

HIV-1

Humans

Male

Mutation

Phylogeny

Plasma Cells

Sequence Analysis, DNA

Viral Load

Viremia

Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated.

Journal article

Gao, Feng|0000-0001-8903-0203

Montefiori, David C|0000-0003-0856-6319

Alam, S Munir|0000-0003-0941-0703

Kelsoe, Garnett|0000-0002-8770-040X

Tomaras, Georgia D|0000-0001-8076-1931

Moody, M Anthony|0000-0002-3890-5855

https://www.ncbi.nlm.nih.gov/pubmed/21987658

2237

2249

11

Basic Science Departments

Clinical Science Departments

Duke

Duke Cancer Institute

Duke Human Vaccine Institute

Faculty

Immunology

Institutes and Centers

Medicine

Medicine, Duke Human Vaccine Institute

Medicine, Infectious Diseases

Molecular Genetics and Microbiology

Pathology

Pediatrics

Pediatrics, Infectious Diseases

School of Medicine

Staff

Surgery

Surgery, Surgical Sciences

Published

208