Capturing and Manipulating Activated Neuronal Ensembles with CANE Delineates a Hypothalamic Social-Fear Circuit
| dc.contributor.author | Sakurai, Katsuyasu | |
| dc.contributor.author | Zhao, Shengli | |
| dc.contributor.author | Takatoh, Jun | |
| dc.contributor.author | Rodriguez, Erica | |
| dc.contributor.author | Lu, Jinghao | |
| dc.contributor.author | Leavitt, Andrew D | |
| dc.contributor.author | Fu, Min | |
| dc.contributor.author | Han, Bao-Xia | |
| dc.contributor.author | Wang, Fan | |
| dc.date.accessioned | 2016-12-15T19:40:49Z | |
| dc.date.issued | 2016-11-23 | |
| dc.description.abstract | © 2016 Elsevier Inc.We developed a technology (capturing activated neuronal ensembles [CANE]) to label, manipulate, and transsynaptically trace neural circuits that are transiently activated in behavioral contexts with high efficiency and temporal precision. CANE consists of a knockin mouse and engineered viruses designed to specifically infect activated neurons. Using CANE, we selectively labeled neurons that were activated by either fearful or aggressive social encounters in a hypothalamic subnucleus previously known as a locus for aggression, and discovered that social-fear and aggression neurons are intermixed but largely distinct. Optogenetic stimulation of CANE-captured social-fear neurons (SFNs) is sufficient to evoke fear-like behaviors in normal social contexts, whereas silencing SFNs resulted in reduced social avoidance. CANE-based mapping of axonal projections and presynaptic inputs to SFNs further revealed a highly distributed and recurrent neural network. CANE is a broadly applicable technology for dissecting causality and connectivity of spatially intermingled but functionally distinct ensembles. | |
| dc.identifier.eissn | 1097-4199 | |
| dc.identifier.issn | 0896-6273 | |
| dc.identifier.uri | ||
| dc.publisher | Elsevier BV | |
| dc.relation.ispartof | Neuron | |
| dc.relation.isversionof | 10.1016/j.neuron.2016.10.015 | |
| dc.title | Capturing and Manipulating Activated Neuronal Ensembles with CANE Delineates a Hypothalamic Social-Fear Circuit | |
| dc.type | Journal article | |
| duke.contributor.orcid | Wang, Fan|0000-0003-2988-0614 | |
| pubs.begin-page | 739 | |
| pubs.end-page | 753 | |
| pubs.issue | 4 | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Cell Biology | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Duke Institute for Brain Sciences | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | Neurobiology | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | University Institutes and Centers | |
| pubs.publication-status | Published | |
| pubs.volume | 92 |