Principles that Govern Competition or Co-existence in Rho-GTPase Driven Polarization
| dc.contributor.advisor | Lew, Daniel J | |
| dc.contributor.author | Chiou, Jian-geng | |
| dc.date.accessioned | 2019-06-07T19:49:15Z | |
| dc.date.available | 2021-05-21T08:17:23Z | |
| dc.date.issued | 2019 | |
| dc.department | Genetics and Genomics | |
| dc.description.abstract | Rho-GTPases are master regulators of polarity establishment and cell morphology. Positive feedback enables concentration of Rho-GTPases into clusters at the cell cortex, from where they regulate the cytoskeleton. Different cell types reproducibly generate either one (e.g. the front of a migrating cell) or several clusters (e.g. the multiple dendrites of a neuron), but the mechanistic basis for unipolar or multipolar outcomes is unclear. The design principles of Rho-GTPase circuits are captured by two-component reaction-diffusion models based on conserved aspects of Rho-GTPase biochemistry. Some such models display rapid winner-takes-all competition between clusters, yielding a unipolar outcome. Other models allow prolonged co-existence of clusters. We investigate the behavior of a simple class of models and show that while the timescale of competition varies enormously depending on model parameters, a single factor explains a large majority of this variation. The dominant factor concerns the degree to which the maximal active GTPase concentration in a cluster approaches a “saturation point” determined by model parameters. We further show that the Rho-GTPase polarity machinery in the budding yeast S. cerevisiae, which normally generates only one bud through competition, can be manipulated to generate multiple buds in ways consistent with this theoretical framework. We suggest that both saturation and the effect of saturation on competition reflect fundamental properties of the Rho-GTPase polarity machinery, regardless of the specific feedback mechanism, which predict whether the system will generate unipolar or multipolar outcomes. | |
| dc.identifier.uri | ||
| dc.subject | Cellular biology | |
| dc.subject | Budding yeast | |
| dc.subject | Pattern formation | |
| dc.subject | Polarity | |
| dc.subject | Rho-GTPase | |
| dc.subject | Turing | |
| dc.title | Principles that Govern Competition or Co-existence in Rho-GTPase Driven Polarization | |
| dc.type | Dissertation | |
| duke.embargo.months | 23 |