Increase in Free and Total Plasma TGF-β1 Following Physical Activity.
| dc.contributor.author | Han, Ashley J | |
| dc.contributor.author | Alexander, Louie C | |
| dc.contributor.author | Huebner, Janet L | |
| dc.contributor.author | Reed, Alexander B | |
| dc.contributor.author | Kraus, Virginia B | |
| dc.date.accessioned | 2024-02-29T16:14:27Z | |
| dc.date.available | 2024-02-29T16:14:27Z | |
| dc.date.issued | 2021-12 | |
| dc.description.abstract | ObjectiveTo evaluate effects of physical activity and food consumption on plasma concentrations of free and total transforming growth factor beta-1 (TGF-β1), beta-2 (TGF-β2), and beta-3 (TGF-β3) in individuals with knee osteoarthritis (OA).MethodsParticipants (n = 40 in 2 cohorts of 20; mean age 70 years) with radiographic knee OA were admitted overnight for serial blood sampling. Cohorts 1 and 2 assessed the impacts of food intake and activity, respectively, on TGF-β concentrations. Cohort 1 blood draws included 2 hours postprandial the evening of day 1 (T3), fasting before rising on day 2 (T0), nonfasting 1 hour after rising (T1B), and 4 hours after rising (T2). Cohort 2 blood draws included T3, T0, fasting 1 hour after rising and performing activities of daily living (T1A), and nonfasting 2 hours after rising (T1B). By sandwich ELISAs, we quantified plasma free and total TGF-β1 concentrations in all samples, and plasma total TGF-β2 and TGF-β3 in cohort 2.ResultsFree TGF-β1 represented a small fraction of the total systemic concentration (mean 0.026%). In cohort 2, free and total TGF-β1 and total TGF-β2 concentration significantly increased in fasting samples collected after an hour (T1A) of activities of daily living (free TGF-β1: P = 0.006; total TGF-β1: P < 0.001; total TGF-β2: P = 0.001). Total TGF-β3 increased nonsignificantly following activity (P = 0.590) and decreased (P = 0.035) after food consumption while resting (T1B).ConclusionsIncreased plasma concentrations of TGF-β with physical activity suggests activity should be standardized prior to TGF-β1 analyses. | |
| dc.identifier.issn | 1947-6035 | |
| dc.identifier.issn | 1947-6043 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | SAGE Publications | |
| dc.relation.ispartof | Cartilage | |
| dc.relation.isversionof | 10.1177/1947603520916523 | |
| dc.rights.uri | ||
| dc.subject | Humans | |
| dc.subject | Osteoarthritis, Knee | |
| dc.subject | Exercise | |
| dc.subject | Activities of Daily Living | |
| dc.subject | Aged | |
| dc.subject | Transforming Growth Factor beta1 | |
| dc.subject | Transforming Growth Factor beta3 | |
| dc.title | Increase in Free and Total Plasma TGF-β1 Following Physical Activity. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Reed, Alexander B|0000-0003-3340-0214 | |
| duke.contributor.orcid | Kraus, Virginia B|0000-0001-8173-8258 | |
| pubs.begin-page | 1741S | |
| pubs.end-page | 1748S | |
| pubs.issue | 1_suppl | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Staff | |
| pubs.organisational-group | Basic Science Departments | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Biostatistics & Bioinformatics | |
| pubs.organisational-group | Medicine | |
| pubs.organisational-group | Orthopaedic Surgery | |
| pubs.organisational-group | Pathology | |
| pubs.organisational-group | Medicine, Rheumatology and Immunology | |
| pubs.organisational-group | Duke Molecular Physiology Institute | |
| pubs.organisational-group | Regeneration Next Initiative | |
| pubs.publication-status | Published | |
| pubs.volume | 13 |
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