Rab4 and Rab10 Oppositely Regulate AMPA Receptors Exocytosis and Structural Plasticity in Single Dendritic Spines
| dc.contributor.advisor | Yasuda, Ryohei | |
| dc.contributor.author | Wang, Jie | |
| dc.date.accessioned | 2017-01-04T20:34:56Z | |
| dc.date.available | 2018-12-09T09:17:13Z | |
| dc.date.issued | 2016 | |
| dc.department | Neurobiology | |
| dc.description.abstract | Membrane trafficking in dendritic spines is critical for regulating the number of channels and spine structure during synaptic plasticity. Here I report two small Rab GTPases, Rab4 and Rab10, oppositely regulate AMPA receptors (AMPARs) trafficking and structural plasticity of dendritic spines. Combining two-photon glutamate uncaging with two-photon fluorescence lifetime imaging microscopy (2pFLIM), I found that Rab4 is transiently activated whereas Rab10 is persistently inactivated in the stimulated spines during structural long-term potentiation (sLTP). Inhibition of Rab4 signaling has no effect on GluA1 endocytosis but inhibits activity-dependent GluA1 exocytosis. Conversely, disruption of Rab10 signaling inhibits GluA1 endocytosis while enhancing activity-dependent GluA1 exocytosis. In summary, these results uncover a new mechanism to establish the specificity and directionality of AMPARs trafficking and sLTP via distinct regulations of Rab4 and Rab10 signaling. | |
| dc.identifier.uri | ||
| dc.subject | Neurosciences | |
| dc.title | Rab4 and Rab10 Oppositely Regulate AMPA Receptors Exocytosis and Structural Plasticity in Single Dendritic Spines | |
| dc.type | Dissertation | |
| duke.embargo.months | 23 |