Complete genome sequencing and analysis of six enterovirus 71 strains with different clinical phenotypes.
Abstract
BACKGROUND: Hand, foot and mouth diseases (HFMD) caused by enterovirus 71(EV71) presents
a broad spectrum of clinical manifestations ranging from mild febrile disease to fatal
neurolocal disease. However, the mechanism of virulence is unknown. METHODS: We isolated
6 strains of EV71 from HFMD patients with or without neurological symptoms, and sequenced
the whole genomes of the viruses to reveal the virulence factors of EV71. RESULTS:
Phylogenetic tree based on VP1 region showed that all six strains clustered into C4a
of C4 sub-genotype. In the complete polypeptide, 298 positions were found to be variable
in all strains, and three of these positions (Val(P814)/Ile(P814) in VP1, Val(P1148)/Ile(P1148)
in 3A and Ala(P1728)/Cys)/Val(P1728) in 3C) were conserved among the strains with
neurovirulence, but variable in strains without neurovirulence. In the 5'-UTR region,
it showed that the first 10 nucleotides were mostly conserved, however from the 11th
nucleotide, nucleotide insertions and deletions were quite common. The secondary structure
prediction of 5'-UTR sequences showed that two of three strains without neurovirulence
(SDLY11 and SDLY48) were almost the same, and all strains with neurovirulence (SDLY96,
SDLY107 and SDLY153) were different from each other. SDLY107 (a fatal strain) was
found different from other strains on four positions (C(P241)/T(P241), A(P571)/T(P571),
C(P579)/T(P579) in 5'-UTR and T(P7335)/C(P7335) in 3'-UTR). CONCLUSIONS: The three
positions (Val(P814)/Ile(P814) in VP1, Val(P1148)/Ile(P1148) in 3A and Ala(P1728)/Cys(P1728)/Val(P1728)
in 3C), were different between two phenotypes. These suggested that the three positions
might be potential virulent positions. And the three varied positions were also found
to be conserved in strains with neurovirulence, and variable in strains without neurovirulence.
These might reveal that the conservation of two of the three positions or the three
together were specific for the strains with neurovirulence. Varation of secondary
structure of 5'-UTR, might be correlated to the changes of viral virulence. SDLY107
(a fatal strain) was found different from other strains on four positions, these positions
might be related with death.
Type
Journal articleSubject
Amino Acid SubstitutionCluster Analysis
Enterovirus A, Human
Genome, Viral
Genotype
Hand, Foot and Mouth Disease
Humans
Molecular Sequence Data
Phylogeny
RNA, Viral
Sequence Analysis, DNA
Viral Proteins
Virulence
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https://hdl.handle.net/10161/13591Published Version (Please cite this version)
10.1186/1743-422X-10-115Publication Info
Wen, Hong-ling; Si, Lu-ying; Yuan, Xiao-jing; Hao, Shu-bin; Gao, Feng; Chu, Fu-lu;
... Wang, Zhi-yu (2013). Complete genome sequencing and analysis of six enterovirus 71 strains with different
clinical phenotypes. Virol J, 10. pp. 115. 10.1186/1743-422X-10-115. Retrieved from https://hdl.handle.net/10161/13591.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Feng Gao
Professor Emeritus in Medicine
Dr. Feng Gao is Professor of Medicine at Duke University. The Gao laboratory has a
long-standing interest in elucidating the origins and evolution of human and simian
inmmunodeficiency viruses (HIV and SIV), and in studying HIV/SIV gene function and
pathogenic mechanisms from the evolutionary perspective. These studies have led to
new strategies to better understand HIV origins, biology, pathogenesis and drug resistance,
and to design new AIDS vaccines.

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