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Computed tomography imaging of primary lung cancer in mice using a liposomal-iodinated contrast agent.

dc.contributor.author Annapragada, A
dc.contributor.author Athreya, KK
dc.contributor.author Badea, CT
dc.contributor.author Clark, D
dc.contributor.author Espinosa, G
dc.contributor.author Ghafoori, AP
dc.contributor.author Ghaghada, KB
dc.contributor.author Johnson, G Allan
dc.contributor.author Kirsch, David Guy
dc.contributor.author Li, Y
dc.coverage.spatial United States
dc.date.accessioned 2018-03-05T22:38:22Z
dc.date.available 2018-03-05T22:38:22Z
dc.date.issued 2012
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/22485175
dc.identifier PONE-D-11-22697
dc.identifier.uri http://hdl.handle.net/10161/16171
dc.description.abstract PURPOSE: To investigate the utility of a liposomal-iodinated nanoparticle contrast agent and computed tomography (CT) imaging for characterization of primary nodules in genetically engineered mouse models of non-small cell lung cancer. METHODS: Primary lung cancers with mutations in K-ras alone (Kras(LA1)) or in combination with p53 (LSL-Kras(G12D);p53(FL/FL)) were generated. A liposomal-iodine contrast agent containing 120 mg Iodine/mL was administered systemically at a dose of 16 µl/gm body weight. Longitudinal micro-CT imaging with cardio-respiratory gating was performed pre-contrast and at 0 hr, day 3, and day 7 post-contrast administration. CT-derived nodule sizes were used to assess tumor growth. Signal attenuation was measured in individual nodules to study dynamic enhancement of lung nodules. RESULTS: A good correlation was seen between volume and diameter-based assessment of nodules (R(2)>0.8) for both lung cancer models. The LSL-Kras(G12D);p53(FL/FL) model showed rapid growth as demonstrated by systemically higher volume changes compared to the lung nodules in Kras(LA1) mice (p<0.05). Early phase imaging using the nanoparticle contrast agent enabled visualization of nodule blood supply. Delayed-phase imaging demonstrated significant differential signal enhancement in the lung nodules of LSL-Kras(G12D);p53(FL/FL) mice compared to nodules in Kras(LA1) mice (p<0.05) indicating higher uptake and accumulation of the nanoparticle contrast agent in rapidly growing nodules. CONCLUSIONS: The nanoparticle iodinated contrast agent enabled visualization of blood supply to the nodules during the early-phase imaging. Delayed-phase imaging enabled characterization of slow growing and rapidly growing nodules based on signal enhancement. The use of this agent could facilitate early detection and diagnosis of pulmonary lesions as well as have implications on treatment response and monitoring.
dc.language eng
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0034496
dc.subject Animals
dc.subject Carcinoma, Non-Small-Cell Lung
dc.subject Contrast Media
dc.subject Liposomes
dc.subject Lung Neoplasms
dc.subject Mice
dc.subject Mice, Transgenic
dc.subject Nanoparticles
dc.subject Proto-Oncogene Proteins p21(ras)
dc.subject Tomography, X-Ray Computed
dc.subject Triiodobenzoic Acids
dc.subject Tumor Burden
dc.subject Tumor Suppressor Protein p53
dc.title Computed tomography imaging of primary lung cancer in mice using a liposomal-iodinated contrast agent.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/22485175
pubs.begin-page e34496
pubs.issue 4
pubs.organisational-group Basic Science Departments
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Pharmacology & Cancer Biology
pubs.organisational-group Physics
pubs.organisational-group Radiation Oncology
pubs.organisational-group Radiology
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 7
dc.identifier.eissn 1932-6203


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