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IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells.

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Date
2018-01
Authors
Blasi, Maria
Negri, Donatella
LaBranche, Celia
Alam, S Munir
Baker, Erich J
Brunner, Elizabeth C
Gladden, Morgan A
Michelini, Zuleika
Vandergrift, Nathan A
Wiehe, Kevin J
Parks, Robert
Shen, Xiaoying
Bonsignori, Mattia
Tomaras, Georgia D
Ferrari, Guido
Montefiori, David C
Santra, Sampa
Haynes, Barton F
Moody, Michael A
Cara, Andrea
Klotman, Mary E
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(21 total)
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Abstract
HIV continues to be a major global health issue. In spite of successful prevention interventions and treatment methods, the development of an HIV vaccine remains a major priority for the field and would be the optimal strategy to prevent new infections. We showed previously that a single immunization with a SIV-based integrase-defective lentiviral vector (IDLV) expressing the 1086.C HIV-1-envelope induced durable, high-magnitude immune responses in non-human primates (NHPs). In this study, we have further characterized the humoral responses by assessing antibody affinity maturation and antigen-specific memory B-cell persistence in two vaccinated macaques. These animals were also boosted with IDLV expressing the heterologous 1176.C HIV-1-Env to determine if neutralization breadth could be increased, followed by evaluation of the injection sites to assess IDLV persistence. IDLV-Env immunization was associated with persistence of the vector DNA for up to 6 months post immunization and affinity maturation of antigen-specific memory B cells.
Type
Journal article
Permalink
https://hdl.handle.net/10161/17606
Published Version (Please cite this version)
10.1038/s42003-018-0131-6
Publication Info
Blasi, Maria; Negri, Donatella; LaBranche, Celia; Alam, S Munir; Baker, Erich J; Brunner, Elizabeth C; ... Klotman, Mary E (2018). IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells. Communications biology, 1. pp. 134. 10.1038/s42003-018-0131-6. Retrieved from https://hdl.handle.net/10161/17606.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Blasi

Maria Blasi

Assistant Professor in Medicine
Dr. Blasi completed her undergraduate and Ph.D. studies in Italy at the Sapienza University in Rome. She moved to Duke in 2012 for a postdoctoral position in Mary Klotman’s laboratory at the Duke Human Vaccine Institute (DHVI).  The Blasi laboratory has two main areas of research: 1) understanding the mechanisms and implications of viral infections in the kidney, including HIV and SARS-CoV-2 and 2) development of vaccines and therapeutics against a variety of infectious dis
Bonsignori

Mattia Bonsignori

Associate Professor in Medicine
HIV vaccine development Study of B-cell immune responses in HIV positive individuals Determination of correlates of protective immunity to HIV Induction of broadly neutralizing antibodies to HIV Development of multiplex functional assays for the evaluation at a single-cell level of B-cell responses to vaccinations, infections and in vitro stimulation Epidemiology and characterization of bacterial resistance determinants (past) </do
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.

Andrea Cara

Visiting Scholar in the Department of Medicine
Ferrari

Guido Ferrari

Professor in Surgery
The activities of the Ferrari Laboratory are based on both independent basic research and immune monitoring studies. The research revolves around three main areas of interest: class I-mediated cytotoxic CD8+ T cell responses, antibody-dependent cellular cytotoxicity (ADCC), gene expression in NK and T cellular subsets upon infection with HIV-1. With continuous funding over the last 11 years from the NIH and Bill & Melinda Gates Foundation along with many other productive collaborations wi
Haynes

Barton Ford Haynes

Frederic M. Hanes Distinguished Professor of Medicine
The Haynes lab is studying host innate and adaptive immune responses to the human immunodeficiency virus (HIV), tuberculosis (TB), and influenza in order to find the enabling technology to make preventive vaccines against these three major infectious diseases. Mucosal Immune Responses in Acute HIV Infection The Haynes lab is working to determine why broadly neutralizing antibodies are rarely made in acute HIV infection (AHI), currently a major obstacle in the de
Klotman

Mary E Klotman

R.J. Reynolds Distinguished Professor of Medicine
Dr. Klotman became Dean of the Duke School of Medicine in June 2017.An accomplished clinician and scientist, Klotman’s research interests are focused on the molecular pathogenesis of Human Immunodeficiency Virus 1 (HIV-1) infection.Among many important contributions to this field, Klotman and her team demonstrated that HIV resides in and evolves separately in kidney cells, a critical step in HIV-associated kidney disease. Her research group also has determi
LaBranche

Celia Crane LaBranche

Associate Professor Emeritus
Montefiori

David Charles Montefiori

Professor in Surgery
Dr. Montefiori is Professor and Director of the Laboratory for AIDS Vaccine Research and Development in the Department of Surgery, Division of Surgical Sciences, Duke University Medical Center. His major research interests are viral immunology and AIDS vaccine development, with a special emphasis on neutralizing antibodies. One of his highest priorities is to identify immunogens that generate broadly cross-reactive neutralizing antibodies for inclusion in HIV vaccines.  Many aspects of the
Moody

Michael Anthony Moody

Professor of Pediatrics
Tony Moody, MD is a Professor in the Department of Pediatrics, Division of Infectious Diseases and Professor in the Department of Immunology at Duke University Medical Center. Research in the Moody lab is focused on understanding the B cell responses during infection, vaccination, and disease. The lab has become a resource for human phenotyping, flow characterization, staining and analysis at the Duke Human Vaccine Institute (DHVI). The Moody lab is currently funded to study influenza, syphil
Shen

Xiaoying Shen

Associate Professor in Surgery
Tomaras

Georgia Doris Tomaras

Professor in Surgery
Dr. Georgia Tomaras is a tenured Professor of Surgery, Professor of Immunology, Professor of Molecular Genetics and Microbiology and is a Fellow of the American Academy of Microbiology (AAM) and a Fellow of the American Association for the Advancement of Science (AAAS).  Dr. Tomaras is the Director of the Duke Center for AIDS Research, a founding Director of the Duke Center for Human Systems Immunology, the Director of Lab Science and mPI for the HIV Vaccine Trials Ne
Vandergrift

Nathan A. Vandergrift

Associate Professor in Medicine
Wiehe

Kevin J Wiehe

Associate Professor in Medicine
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Alphabetical list of authors with Scholars@Duke profiles.
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