IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells.
Abstract
HIV continues to be a major global health issue. In spite of successful prevention
interventions and treatment methods, the development of an HIV vaccine remains a major
priority for the field and would be the optimal strategy to prevent new infections.
We showed previously that a single immunization with a SIV-based integrase-defective
lentiviral vector (IDLV) expressing the 1086.C HIV-1-envelope induced durable, high-magnitude
immune responses in non-human primates (NHPs). In this study, we have further characterized
the humoral responses by assessing antibody affinity maturation and antigen-specific
memory B-cell persistence in two vaccinated macaques. These animals were also boosted
with IDLV expressing the heterologous 1176.C HIV-1-Env to determine if neutralization
breadth could be increased, followed by evaluation of the injection sites to assess
IDLV persistence. IDLV-Env immunization was associated with persistence of the vector
DNA for up to 6 months post immunization and affinity maturation of antigen-specific
memory B cells.
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https://hdl.handle.net/10161/17606Published Version (Please cite this version)
10.1038/s42003-018-0131-6Publication Info
(2018). IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific
memory B cells. Communications biology, 1. pp. 134. 10.1038/s42003-018-0131-6. Retrieved from https://hdl.handle.net/10161/17606.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Maria Blasi
Assistant Professor in Medicine
Dr. Blasi completed her undergraduate and Ph.D. studies in Italy at the Sapienza University
in Rome. She moved to Duke for a postdoctoral position in Mary Klotman’s laboratory
at the Duke Human Vaccine Institute. Her research interests focus on two major priorities
in the HIV-1 field: vaccine development and latent reservoirs/ cure studies.
Dr. Blasi’s work encompasses several aspects of HIV-1 research, ranging from designing
and testing a novel vaccine platform base
Mattia Bonsignori
Associate Professor in Medicine
HIV vaccine development Study of B-cell immune responses in HIV positive individuals
Determination of correlates of protective immunity to HIV Induction of broadly neutralizing
antibodies to HIV Development of multiplex functional assays for the evaluation at
a single-cell level of B-cell responses to vaccinations, infections and in vitro stimulation
Epidemiology and characterization of bacterial resistance determinants (past) </do
Andrea Cara
Visiting Scholar in the Department of Medicine
Guido Ferrari
Associate Professor of Surgery
The activities of the Ferrari Laboratory are based on both independent basic research
and immune monitoring studies. The research revolves around three main areas of interest:
class I-mediated cytotoxic CD8+ T cell responses, antibody-dependent cellular cytotoxicity
(ADCC), gene expression in NK and T cellular subsets upon infection with HIV-1. With
continuous funding over the last 11 years from the NIH and Bill & Melinda Gates Foundation
along with many other productive collaborations wi
Barton Ford Haynes
Frederic M. Hanes Distinguished Professor of Medicine
The Haynes lab is studying host innate and adaptive immune responses to the human
immunodeficiency virus (HIV), tuberculosis (TB), and influenza in order to find the
enabling technology to make preventive vaccines against these three major infectious
diseases. Mucosal Immune Responses in Acute HIV Infection The Haynes lab is working
to determine why broadly neutralizing antibodies are rarely made in acute HIV infection
(AHI), currently a major obstacle in the de
Mary E Klotman
R.J. Reynolds Distinguished Professor of Medicine
Dr. Klotman became dean of the Duke School of Medicine in June 2017.An accomplished
clinician and scientist, Klotman’s research interests are focused on the molecular
pathogenesis of Human Immunodeficiency Virus 1 (HIV-1) infection.Among many important
contributions to this field, Klotman and her team demonstrated that HIV resides in
and evolves separately in kidney cells, a critical step in HIV-associated kidney disease.
Her research group also has determi
Celia Crane LaBranche
Associate Professor Emeritus
David Charles Montefiori
Professor of Surgery
Dr. Montefiori is Professor and Director of the Laboratory for AIDS Vaccine Research
and Development in the Department of Surgery, Division of Surgical Sciences, Duke
University Medical Center. His major research interests are viral immunology and AIDS
vaccine development, with a special emphasis on neutralizing antibodies. One of his
highest priorities is to identify immunogens that generate broadly cross-reactive
neutralizing antibodies for inclusion in HIV vaccines. Many aspects of the
Michael Anthony Moody
Associate Professor of Pediatrics
Tony Moody, MD is an Associate Professor in the Department of Pediatrics, Division
of Infectious Diseases and the Department of Immunology at Duke University Medical
Center. Research in the Moody lab is focused on understanding the B cell responses
during infection, vaccination, and disease. The lab has become a resource for human
phenotyping, flow characterization, staining and analysis at the Duke Human Vaccine
Institute (DHVI). The Moody lab is currently funded to study influenza, syphilis
Xiaoying Shen
Associate Professor in Surgery
Georgia Doris Tomaras
Professor in Surgery
Research in the Tomaras Laboratory in the Duke Human Vaccine Institute and Departments
of Surgery, Immunology, and Molecular Genetics and Microbiology at Duke University
Medical Center, focuses on the identification of immune correlates of protection for
preventative vaccines and identification of the mechanisms responsible for potent
inhibition of human pathogens.
Nathan A. Vandergrift
Associate Professor in Medicine
Kevin J Wiehe
Associate Professor in Medicine
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