Associations between expression levels of nucleotide excision repair proteins in lymphoblastoid cells and risk of squamous cell carcinoma of the head and neck.
Abstract
Squamous cell carcinoma of head and neck (SCCHN) is one of the most common malignancies
worldwide, and nucleotide excision repair (NER) is involved in SCCHN susceptibility.
In this analysis of 349 newly diagnosed SCCHN patients and 295 cancer-free controls,
we investigated whether expression levels of eight core NER proteins were associated
with risk of SCCHN. We quantified NER protein expression levels in cultured peripheral
lymphocytes using a reverse-phase protein microarray. Compared with the controls,
SCCHN patients had statistically significantly lower expression levels of ERCC3 and
XPA (P = 0.001 and 0.001, respectively). After dividing the subjects by controls'
median values of expression levels, we found a dose-dependent association between
an increased risk of SCCHN and low expression levels of ERCC3 (adjusted OR, 1.75,
and 95% CI: 1.26-2.42; Ptrend = 0.008) and XPA (adjusted OR, 1.88; 95% CI, 1.35-2.60;
Ptrend = 0.001). We also identified a significant multiplicative interaction between
smoking status and ERCC3 expression levels (P = 0.014). Finally, after integrating
demographic and clinical variables, we found that the addition of ERCC3 and XPA expression
levels to the model significantly improved the sensitivity of the expanded model on
SCCHN risk. In conclusion, reduced protein expression levels of ERCC3 and XPA were
associated with an increased risk of SCCHN. However, these results need to be confirmed
in additional large studies.
Type
Journal articleSubject
LymphocytesCells, Cultured
Humans
Carcinoma, Squamous Cell
Head and Neck Neoplasms
DNA Helicases
DNA-Binding Proteins
Risk Assessment
Risk Factors
Adult
Aged
Aged, 80 and over
Middle Aged
Female
Male
Xeroderma Pigmentosum Group A Protein
Young Adult
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https://hdl.handle.net/10161/18510Published Version (Please cite this version)
10.1002/mc.22801Publication Info
Han, Peng; Liu, Hongliang; Shi, Qiong; Liu, Zhensheng; Troy, Jesse D; Lee, Walter
T; ... Wei, Qingyi (2018). Associations between expression levels of nucleotide excision repair proteins in lymphoblastoid
cells and risk of squamous cell carcinoma of the head and neck. Molecular carcinogenesis, 57(6). pp. 784-793. 10.1002/mc.22801. Retrieved from https://hdl.handle.net/10161/18510.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Walter T Lee
Professor of Head and Neck Surgery and Communication Sciences
Global Health, Virtue-Based Professional Development and Leadership, Device Development
for Cancer Detection
Zhensheng Liu
Assistant Professor of Medicine
Jesse David Troy
Assistant Professor of Biostatistics and Bioinformatics
I am a biostatistician who provides statistical support for research studies in stem
cell transplantation and cell therapies for a variety of indications including cerebral
palsy (CP), brain injury, autism, cancer, and stroke. My personal research interests
are related to medical decision making and patient-centered outcomes in hematologic
malignancies. I recently served as the Scientific Director of the Data Coordinating
Center for the National MDS Study (http://www.thenationalmds
Qingyi Wei
Professor in Population Health Sciences
Qingyi Wei, MD, PhD, Professor in the Department of Medicine, is Associate Director
for Cancer Control and Population Sciences, Co-leader of CCPS and Co-leader of Epidemiology
and Population Genomics (Focus Area 1). He is a professor of Medicine and an internationally
recognized epidemiologist focused on the molecular and genetic epidemiology of head
and neck cancers, lung cancer, and melanoma. His research focuses on biomarkers and
genetic determinants for the DNA repair deficient phenotype and
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