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Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults.

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Date
2021-02-04
Authors
Terrando, Niccolò
Park, John J
Devinney, Michael
Chan, Cliburn
Cooter, Mary
Avasarala, Pallavi
Mathew, Joseph P
Quinones, Quintin J
Maddipati, Krishna Rao
Berger, Miles
MADCO-PC Study Team
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(11 total)
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Abstract
Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation is thought to play a causal role in perioperative neurocognitive disorders, yet the role of these lipids in the human central nervous system in such disorders is unclear. Here we used liquid chromatography-mass spectrometry to quantify AA, DHA, and EPA derived lipid levels in non-centrifuged cerebrospinal fluid (CSF), centrifuged CSF pellets, and centrifuged CSF supernatants of older adults obtained before, 24 h and 6 weeks after surgery. GAGE analysis was used to determine AA, DHA and EPA metabolite pathway changes over time. Lipid mediators derived from AA, DHA and EPA were detected in all sample types. Postoperative lipid mediator changes were not significant in non-centrifuged CSF (p > 0.05 for all three pathways). The AA metabolite pathway showed significant changes in centrifuged CSF pellets and supernatants from before to 24 h after surgery (p = 0.0000247, p = 0.0155 respectively), from before to 6 weeks after surgery (p = 0.0000497, p = 0.0155, respectively), and from 24 h to 6 weeks after surgery (p = 0.0000499, p = 0.00363, respectively). These findings indicate that AA, DHA, and EPA derived lipids are detectable in human CSF, and the AA metabolite pathway shows postoperative changes in centrifuged CSF pellets and supernatants.
Type
Journal article
Subject
MADCO-PC Study Team
Central Nervous System
Humans
Inflammation
Lipids
Docosahexaenoic Acids
Eicosapentaenoic Acid
Arachidonic Acid
Immunologic Factors
Chromatography, Liquid
Aged
Aged, 80 and over
Middle Aged
Female
Male
Lipid Metabolism
Mass Spectrometry
Neurocognitive Disorders
Perioperative Medicine
Permalink
https://hdl.handle.net/10161/24175
Published Version (Please cite this version)
10.1038/s41598-021-82606-5
Publication Info
Terrando, Niccolò; Park, John J; Devinney, Michael; Chan, Cliburn; Cooter, Mary; Avasarala, Pallavi; ... MADCO-PC Study Team (2021). Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults. Scientific reports, 11(1). pp. 3047. 10.1038/s41598-021-82606-5. Retrieved from https://hdl.handle.net/10161/24175.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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Scholars@Duke

Berger

Miles Berger

Associate Professor of Anesthesiology
My research team focuses on understanding the cause of postoperative cognitive dysfunction (POCD) and delirium, and whether these disorders are caused by perioperative changes in Alzheimer's disease pathways. We are also interested in whether delirium or POCD are associated with an increased long term risk of developing Alzheimer's disease. Towards these ends, we use a combination of methods including cognitive testing, CSF and blood sampling, functional neuroimaging, and rigorous biochemical as
Chan

Chi Wei Cliburn Chan

Associate Professor of Biostatistics & Bioinformatics
Computational immunology (stochastic and spatial models and simulations, T cell signaling, immune regulation) Statistical methodology for immunological laboratory techniques (flow cytometry, CFSE analysis, receptor-ligand binding and signaling kinetics) Informatics of the immune system (reference and application ontologies, meta-programming, text mining and machine learning)
Devinney

Michael Devinney

Assistant Professor of Anesthesiology
Delirium and cognitive dysfunction following surgery are associated with significant long-term risk for dementia, diminished quality of life, and increased mortality. Yet, few interventions exist to prevent these neurocognitive disorders, in part due to our poor understanding of their pathophysiologic underpinnings and risk factors. One possible risk factor is obstructive sleep apnea (OSA), a disorder characterized by repeated breathing interruptions during sleep that is highly prevalent yet fre
Mathew

Joseph P. Mathew

Jerry Reves, M.D. Distinguished Professor of Cardiac Anesthesiology
Current research interests include:1. The relationship between white matter patency, functional connectivity (fMRI) and neurocognitive function following cardiac surgery.2. The relationship between global and regional cortical beta-amyloid deposition and postoperative cognitive decline.3. The effect of lidocaine infusion upon neurocognitive function following cardiac surgery.4. The association between genotype and outcome after cardiac surgery.5. Atrial fibrillation
Quinones

Quintin Jose Quinones

Assistant Professor of Anesthesiology
Terrando

Niccolò Terrando

Associate Professor of Anesthesiology
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