Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery in older adults.
Abstract
Arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA)
derived lipids play key roles in initiating and resolving inflammation. Neuro-inflammation
is thought to play a causal role in perioperative neurocognitive disorders, yet the
role of these lipids in the human central nervous system in such disorders is unclear.
Here we used liquid chromatography-mass spectrometry to quantify AA, DHA, and EPA
derived lipid levels in non-centrifuged cerebrospinal fluid (CSF), centrifuged CSF
pellets, and centrifuged CSF supernatants of older adults obtained before, 24 h and
6 weeks after surgery. GAGE analysis was used to determine AA, DHA and EPA metabolite
pathway changes over time. Lipid mediators derived from AA, DHA and EPA were detected
in all sample types. Postoperative lipid mediator changes were not significant in
non-centrifuged CSF (p > 0.05 for all three pathways). The AA metabolite pathway showed
significant changes in centrifuged CSF pellets and supernatants from before to 24 h
after surgery (p = 0.0000247, p = 0.0155 respectively), from before to 6 weeks after
surgery (p = 0.0000497, p = 0.0155, respectively), and from 24 h to 6 weeks after
surgery (p = 0.0000499, p = 0.00363, respectively). These findings indicate that AA,
DHA, and EPA derived lipids are detectable in human CSF, and the AA metabolite pathway
shows postoperative changes in centrifuged CSF pellets and supernatants.
Type
Journal articleSubject
MADCO-PC Study TeamCentral Nervous System
Humans
Inflammation
Lipids
Docosahexaenoic Acids
Eicosapentaenoic Acid
Arachidonic Acid
Immunologic Factors
Chromatography, Liquid
Aged
Aged, 80 and over
Middle Aged
Female
Male
Lipid Metabolism
Mass Spectrometry
Neurocognitive Disorders
Perioperative Medicine
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https://hdl.handle.net/10161/24175Published Version (Please cite this version)
10.1038/s41598-021-82606-5Publication Info
Terrando, Niccolò; Park, John J; Devinney, Michael; Chan, Cliburn; Cooter, Mary; Avasarala,
Pallavi; ... MADCO-PC Study Team (2021). Immunomodulatory lipid mediator profiling of cerebrospinal fluid following surgery
in older adults. Scientific reports, 11(1). pp. 3047. 10.1038/s41598-021-82606-5. Retrieved from https://hdl.handle.net/10161/24175.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Miles Berger
Associate Professor of Anesthesiology
My research team focuses on understanding the cause of postoperative cognitive dysfunction
(POCD) and delirium, and whether these disorders are caused by perioperative changes
in Alzheimer's disease pathways. We are also interested in whether delirium or POCD
are associated with an increased long term risk of developing Alzheimer's disease.
Towards these ends, we use a combination of methods including cognitive testing, CSF
and blood sampling, functional neuroimaging, and rigorous biochemical as
Chi Wei Cliburn Chan
Associate Professor of Biostatistics & Bioinformatics
Computational immunology (stochastic and spatial models and simulations, T cell signaling,
immune regulation) Statistical methodology for immunological laboratory techniques
(flow cytometry, CFSE analysis, receptor-ligand binding and signaling kinetics) Informatics
of the immune system (reference and application ontologies, meta-programming, text
mining and machine learning)
Michael Devinney
Assistant Professor of Anesthesiology
Delirium and cognitive dysfunction following surgery are associated with significant
long-term risk for dementia, diminished quality of life, and increased mortality.
Yet, few interventions exist to prevent these neurocognitive disorders, in part due
to our poor understanding of their pathophysiologic underpinnings and risk factors.
One possible risk factor is obstructive sleep apnea (OSA), a disorder characterized
by repeated breathing interruptions during sleep that is highly prevalent yet fre
Joseph P. Mathew
Jerry Reves, M.D. Distinguished Professor of Cardiac Anesthesiology
Current research interests include:1. The relationship between white matter patency,
functional connectivity (fMRI) and neurocognitive function following cardiac surgery.2.
The relationship between global and regional cortical beta-amyloid deposition and
postoperative cognitive decline.3. The effect of lidocaine infusion upon neurocognitive
function following cardiac surgery.4. The association between genotype and outcome
after cardiac surgery.5. Atrial fibrillation
Quintin Jose Quinones
Assistant Professor of Anesthesiology
Niccolò Terrando
Associate Professor of Anesthesiology
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