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Mycoplasma-related endobacteria within Mortierellomycotina fungi: diversity, distribution and functional insights into their lifestyle.

dc.contributor.author Desirò, Alessandro
dc.contributor.author Hao, Zhen
dc.contributor.author Liber, Julian A
dc.contributor.author Benucci, Gian Maria Niccolò
dc.contributor.author Lowry, David
dc.contributor.author Roberson, Robert
dc.contributor.author Bonito, Gregory
dc.date.accessioned 2022-01-14T15:41:32Z
dc.date.available 2022-01-14T15:41:32Z
dc.date.issued 2018-06
dc.identifier 10.1038/s41396-018-0053-9
dc.identifier.issn 1751-7362
dc.identifier.issn 1751-7370
dc.identifier.uri https://hdl.handle.net/10161/24217
dc.description.abstract Bacterial interactions with animals and plants have been examined for over a century; by contrast, the study of bacterial-fungal interactions has received less attention. Bacteria interact with fungi in diverse ways, and endobacteria that reside inside fungal cells represent the most intimate interaction. The most significant bacterial endosymbionts that have been studied are associated with Mucoromycota and include two main groups: Burkholderia-related and Mycoplasma-related endobacteria (MRE). Examples of Burkholderia-related endobacteria have been reported in the three Mucoromycota subphyla. By contrast, MRE have only been identified in Glomeromycotina and Mucoromycotina. This study aims to understand whether MRE dwell in Mortierellomycotina and, if so, to determine their impact on the fungal host. We carried out a large-scale screening of 394 Mortierellomycotina strains and employed a combination of microscopy, molecular phylogeny, next-generation sequencing and qPCR. We detected MRE in 12 strains. These endosymbionts represent novel bacterial phylotypes and show evidence of recombination. Their presence in Mortierellomycotina demonstrates that MRE occur within fungi across Mucoromycota and they may have lived in their common ancestor. We cured the fungus of its endosymbionts with antibiotics and observed improved biomass production in isogenic lines lacking MRE, demonstrating that these endobacteria impose some fitness costs to their fungal host. Here we provided the first functional insights into the lifestyle of MRE. Our findings indicate that MRE may be antagonistic to their fungal hosts, and adapted to a non-lethal parasitic lifestyle in the mycelium of Mucoromycota. However, context-dependent adaptive benefits to their host at minimal cost cannot not be excluded. Finally, we conclude that Mortierellomycotina represent attractive model organisms for exploring interactions between MRE and fungi.
dc.language eng
dc.publisher Springer Science and Business Media LLC
dc.relation.ispartof The ISME journal
dc.relation.isversionof 10.1038/s41396-018-0053-9
dc.subject Burkholderia
dc.subject Mycoplasma
dc.subject Fungi
dc.subject Biodiversity
dc.subject Phylogeny
dc.subject Symbiosis
dc.title Mycoplasma-related endobacteria within Mortierellomycotina fungi: diversity, distribution and functional insights into their lifestyle.
dc.type Journal article
duke.contributor.id Liber, Julian A|0707293
dc.date.updated 2022-01-14T15:41:31Z
pubs.begin-page 1743
pubs.end-page 1757
pubs.issue 7
pubs.organisational-group Duke
pubs.organisational-group Trinity College of Arts & Sciences
pubs.organisational-group Student
pubs.organisational-group Biology
pubs.publication-status Published
pubs.volume 12
duke.contributor.orcid Liber, Julian A|0000-0002-9941-8268


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