Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor cell products.
Abstract
<h4>Background aims</h4>Removing DMSO post-thaw results in: reduced infusion reactions,
improved recovery and stability of viable CD34+ cells. Validated methods use 5%-8.3%
Dextran 40 with 2.5%-4.2% HSA for this purpose. Recent shortages of clinical grade
Dextran require identification of suitable alternatives.<h4>Methods</h4>PBPC were
used to compare a standard 2X wash medium of 5 parts 10% Dextran 40 in saline (DEX)
with 1 part 25% HSA (8.3% DEX/ 4.2% HSA) with Hydroxyethyl Starch (HES)-based solutions.
Cells in replicate bags were diluted with an equal volume of wash solution, equilibrated
5 minutes, the bag filled with wash medium, pelleted and the supernatant expressed.
Bags were restored to the frozen volume in wash medium and tested by single platform
flow cytometry and CFU. Total viability, viable TNC, MNC, and CD34+ cell recovery,
and CD34+ cell viability were compared immediately post-thaw and after 90 minutes.<h4>Results</h4>5.2%
HES/4.2% HSA did not differ from our standard in CD34 recovery or viability. Due to
concerns that high concentrations of HES could affect renal function we tested 0.6%
HES/2.5% HSA resulting in significantly poorer CD34 recovery and viability. Results
improved using 2.4% HES/4.2% HSA and when 0.6% HES/4.2%HSA was used no significant
differences were seen. CFU assays confirmed no differences between the standard dextran
arm and HES at 2.4% or 0.6% so long as HSA was at 4.2%.<h4>Conclusions</h4>We conclude
that HES from 0.6% to 5.2% with 4.2% HSA is a suitable substitute for Dextran 40 as
a reconstitution/washing medium for PBPC products.
Type
Journal articleSubject
Hematopoietic Stem CellsCells, Cultured
Humans
Dextrans
Culture Media
Cryopreservation
Colony-Forming Units Assay
Cell Survival
Freezing
Male
Hydroxyethyl Starch Derivatives
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https://hdl.handle.net/10161/24615Published Version (Please cite this version)
10.1016/j.jcyt.2015.08.007Publication Info
Zhu, Fenlu; Heditke, Sarah; Kurtzberg, Joanne; Waters-Pick, Barbara; Hari, Parameswaran;
Margolis, David A; & Keever-Taylor, Carolyn A (2015). Hydroxyethyl starch as a substitute for dextran 40 for thawing peripheral blood progenitor
cell products. Cytotherapy, 17(12). pp. 1813-1819. 10.1016/j.jcyt.2015.08.007. Retrieved from https://hdl.handle.net/10161/24615.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Joanne Kurtzberg
Jerome S. Harris Distinguished Professor of Pediatrics
Dr. Kurtzberg conducts both clinical and laboratory-based translational research
efforts, all involving various aspects of normal and malignant hematopoiesis. In the
laboratory, her early work focused on studies determining the mechanisms that regulate
the choice between the various pathways of differentiation available to the pluripotent
hematopoietic stem cell. Her laboratory established a CD7+ cell line, DU.528, capable
of multilineage differentiation as well as self-renewal, and subse

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