Show simple item record Weaver, EA Camacho, ZT Gao, F
dc.coverage.spatial United States 2011-04-15T16:46:19Z 2010-05
dc.identifier.citation AIDS Res Hum Retroviruses, 2010, 26 (5), pp. 577 - 584
dc.description.abstract Consensus HIV-1 genes can decrease the genetic distances between candidate immunogens and field virus strains. To ensure the functionality and optimal presentation of immunologic epitopes, we generated two group-M consensus env genes that contain variable regions either from a wild-type B/C recombinant virus isolate (CON6) or minimal consensus elements (CON-S) in the V1, V2, V4, and V5 regions. C57BL/6 and BALB/c mice were primed twice with CON6, CON-S, and subtype control (92UG37_A and HXB2/Bal_B) DNA and boosted with recombinant vaccinia virus (rVV). Mean antibody titers against 92UG37_A, 89.6_B, 96ZM651_C, CON6, and CON-S Env protein were determined. Both CON6 and CON-S induced higher mean antibody titers against several of the proteins, as compared with the subtype controls. However, no significant differences were found in mean antibody titers in animals immunized with CON6 or CON-S. Cellular immune responses were measured by using five complete Env overlapping peptide sets: subtype A (92UG37_A), subtype B (MN_B, 89.6_B and SF162_B), and subtype C (Chn19_C). The intensity of the induced cellular responses was measured by using pooled Env peptides; T-cell epitopes were identified by using matrix peptide pools and individual peptides. No significant differences in T-cell immune-response intensities were noted between CON6 and CON-S immunized BALB/c and C57BL/6 mice. In BALB/c mice, 10 and eight nonoverlapping T-cell epitopes were identified in CON6 and CON-S, whereas eight epitopes were identified in 92UG37_A and HXB2/BAL_B. In C57BL/6 mice, nine and six nonoverlapping T-cell epitopes were identified after immunization with CON6 and CON-S, respectively, whereas only four and three were identified in 92UG37_A and HXB2/BAL_B, respectively. When combined together from both mouse strains, 18 epitopes were identified. The group M artificial consensus env genes, CON6 and CON-S, were equally immunogenic in breadth and intensity for inducing humoral and cellular immune responses.
dc.format.extent 577 - 584
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof AIDS Res Hum Retroviruses
dc.relation.isversionof 10.1089/aid.2009.0258
dc.subject AIDS Vaccines
dc.subject Amino Acid Sequence
dc.subject Animals
dc.subject Consensus Sequence
dc.subject Epitopes, T-Lymphocyte
dc.subject Female
dc.subject HIV Antibodies
dc.subject HIV Infections
dc.subject HIV-1
dc.subject Immunization
dc.subject Mice
dc.subject Mice, Inbred BALB C
dc.subject Mice, Inbred C57BL
dc.subject Molecular Sequence Data
dc.subject T-Lymphocytes
dc.subject Vaccines, DNA
dc.subject Vaccinia virus
dc.subject env Gene Products, Human Immunodeficiency Virus
dc.title Similar T-cell immune responses induced by group M consensus env immunogens with wild-type or minimum consensus variable regions.
dc.type Journal Article
dc.description.version Version of Record en_US 2010-5-0 en_US
duke.description.endpage 584 en_US
duke.description.issue 5 en_US
duke.description.startpage 577 en_US
duke.description.volume 26 en_US
dc.relation.journal AIDS Research and Human Retroviruses en_US
pubs.issue 5
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Infectious Diseases
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Human Vaccine Institute
pubs.publication-status Published
pubs.volume 26
dc.identifier.eissn 1931-8405

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