Short-lived alpha-helical intermediates in the folding of beta-sheet proteins.
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Several lines of evidence point strongly toward the importance of highly alpha-helical intermediates in the folding of all globular proteins, regardless of their native structure. However, experimental refolding studies demonstrate no observable alpha-helical intermediate during refolding of some beta-sheet proteins and have dampened enthusiasm for this model of protein folding. In this study, beta-sheet proteins were hypothesized to have potential to form amphiphilic helices at a period of <3.6 residues/turn that matches or exceeds the potential at 3.6 residues/turn. Hypothetically, such potential is the basis for an effective and unidirectional mechanism by which highly alpha-helical intermediates might be rapidly disassembled during folding and potentially accounts for the difficulty in detecting highly alpha-helical intermediates during the folding of some proteins. The presence of this potential was confirmed, indicating that a model entailing ubiquitous formation of alpha-helical intermediates during the folding of globular proteins predicts previously unrecognized features of primary structure. Further, the folding of fatty acid binding protein, a predominantly beta-sheet protein that exhibits no apparent highly alpha-helical intermediate during folding, was dramatically accelerated by 2,2,2-trifluoroethanol, a solvent that stabilizes alpha-helical structure. This observation suggests that formation of an alpha-helix can be a rate-limiting step during folding of a predominantly beta-sheet protein and further supports the role of highly alpha-helical intermediates in the folding of all globular proteins.
SubjectFatty Acid-Binding Proteins
Protein Structure, Secondary
Published Version (Please cite this version)10.1021/bi100288q
Publication InfoChen, Eefei; Everett, Mary Lou; Holzknecht, Zoie E; Holzknecht, Robert A; Lin, Shu S; Bowles, Dawn E; & Parker, William (2010). Short-lived alpha-helical intermediates in the folding of beta-sheet proteins. Biochemistry, 49(26). pp. 5609-5619. 10.1021/bi100288q. Retrieved from https://hdl.handle.net/10161/4008.
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Assistant Professor in Surgery
Associate Professor of Surgery
Introduction The increasing number and the improvement in the success of heart and lung transplantation in recent years make now the most exciting time to be involved in research related to the scientific issues surrounding this field. Among those issues, two problems are currently recognized to be the major impediment to the optimal application of transplantation in patients with end-stage cardiopulmonary disease. First, there is a lack of consistent long-term gra
Associate Professor of Surgery
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