Show simple item record Tachibana, H Naga Prasad, SV Lefkowitz, RJ Koch, WJ Rockman, HA
dc.coverage.spatial United States 2012-10-22T20:57:19Z 2005-02-08
dc.identifier 01.CIR.0000142291.70954.DF
dc.identifier.citation Circulation, 2005, 111 (5), pp. 591 - 597
dc.description.abstract BACKGROUND: Heart failure is characterized by abnormalities in beta-adrenergic receptor (betaAR) signaling, including increased level of myocardial betaAR kinase 1 (betaARK1). Our previous studies have shown that inhibition of betaARK1 with the use of the Gbetagamma sequestering peptide of betaARK1 (betaARKct) can prevent cardiac dysfunction in models of heart failure. Because inhibition of betaARK activity is pivotal for amelioration of cardiac dysfunction, we investigated whether the level of betaARK1 inhibition correlates with the degree of heart failure. METHODS AND RESULTS: Transgenic (TG) mice with varying degrees of cardiac-specific expression of betaARKct peptide underwent transverse aortic constriction (TAC) for 12 weeks. Cardiac function was assessed by serial echocardiography in conscious mice, and the level of myocardial betaARKct protein was quantified at termination of the study. TG mice showed a positive linear relationship between the level of betaARKct protein expression and fractional shortening at 12 weeks after TAC. TG mice with low betaARKct expression developed severe heart failure, whereas mice with high betaARKct expression showed significantly less cardiac deterioration than wild-type (WT) mice. Importantly, mice with a high level of betaARKct expression had preserved isoproterenol-stimulated adenylyl cyclase activity and normal betaAR densities in the cardiac membranes. In contrast, mice with low expression of the transgene had marked abnormalities in betaAR function, similar to the WT mice. CONCLUSIONS: These data show that the level of betaARK1 inhibition determines the degree to which cardiac function can be preserved in response to pressure overload and has important therapeutic implications when betaARK1 inhibition is considered as a molecular target.
dc.format.extent 591 - 597
dc.language eng
dc.relation.ispartof Circulation
dc.relation.isversionof 10.1161/01.CIR.0000142291.70954.DF
dc.subject Adenylyl Cyclases
dc.subject Animals
dc.subject Cardiac Output, Low
dc.subject Constriction
dc.subject Cyclic AMP-Dependent Protein Kinases
dc.subject Heart
dc.subject Mice
dc.subject Mice, Transgenic
dc.subject Myocardium
dc.subject Peptides
dc.subject Pressure
dc.subject Recombinant Proteins
dc.subject Signal Transduction
dc.subject Ultrasonography
dc.subject beta-Adrenergic Receptor Kinases
dc.title Level of beta-adrenergic receptor kinase 1 inhibition determines degree of cardiac dysfunction after chronic pressure overload-induced heart failure.
dc.type Journal Article
duke.description.endpage 597 en_US
duke.description.issue 5 en_US
duke.description.startpage 591 en_US
duke.description.volume 111 en_US
dc.relation.journal Circulation en_US
pubs.issue 5
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Biochemistry
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Cell Biology
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Cardiology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Pathology
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.organisational-group /Duke/Trinity College of Arts & Sciences
pubs.organisational-group /Duke/Trinity College of Arts & Sciences/Chemistry
pubs.publication-status Published
pubs.volume 111
dc.identifier.eissn 1524-4539

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