Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ.

dc.contributor.author

Gardner, Kiani AJ Arkus

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Osawa, Masaki

dc.contributor.author

Erickson, Harold P

dc.contributor.editor

Weiss, David

dc.coverage.spatial

United States

dc.date.accessioned

2017-05-15T18:10:02Z

dc.date.available

2017-05-15T18:10:02Z

dc.date.issued

2017

dc.description.abstract

FtsZ is an essential protein for bacterial cell division, where it forms the cytoskeletal scaffold and may generate the constriction force. We have found previously that some mutant and foreign FtsZ that do not complement an ftsZ null can function for cell division in E. coli upon acquisition of a suppressor mutation somewhere in the genome. We have now identified, via whole genome re-sequencing, single nucleotide polymorphisms in 11 different suppressor strains. Most of the mutations are in genes of various metabolic pathways, which may modulate cell division indirectly. Mutations in three genes, ispA, accD and nlpI, may be more directly involved in cell division. In addition to the genomic suppressor mutations, we identified intragenic suppressors of three FtsZ point mutants (R174A, E250K and L272V).

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/28445510

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PONE-D-16-48416

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1932-6203

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https://hdl.handle.net/10161/14347

dc.language

eng

dc.publisher

Public Library of Science (PLoS)

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PLoS One

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10.1371/journal.pone.0176643

dc.title

Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ.

dc.type

Journal article

duke.contributor.orcid

Erickson, Harold P|0000-0002-9104-8987

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/28445510

pubs.begin-page

e0176643

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4

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Basic Science Departments

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Biochemistry

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Cell Biology

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Duke

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Duke Cancer Institute

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Institutes and Centers

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School of Medicine

pubs.publication-status

Published online

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12

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