Epigenetic and transcriptional responses in circulating leukocytes are associated with future decompensation during SARS-CoV-2 infection.
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2024-01
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To elucidate host response elements that define impending decompensation during SARS-CoV-2 infection, we enrolled subjects hospitalized with COVID-19 who were matched for disease severity and comorbidities at the time of admission. We performed combined single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq) on peripheral blood mononuclear cells (PBMCs) at admission and compared subjects who improved from their moderate disease with those who later clinically decompensated and required invasive mechanical ventilation or died. Chromatin accessibility and transcriptomic immune profiles were markedly altered between the two groups, with strong signals in CD4+ T cells, inflammatory T cells, dendritic cells, and NK cells. Multiomic signature scores at admission were tightly associated with future clinical deterioration (auROC 1.0). Epigenetic and transcriptional changes in PBMCs reveal early, broad immune dysregulation before typical clinical signs of decompensation are apparent and thus may act as biomarkers to predict future severity in COVID-19.
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McClain, Micah T, Ilya Zhbannikov, Lisa L Satterwhite, Ricardo Henao, Nicholas S Giroux, Shengli Ding, Thomas W Burke, Ephraim L Tsalik, et al. (2024). Epigenetic and transcriptional responses in circulating leukocytes are associated with future decompensation during SARS-CoV-2 infection. iScience, 27(1). p. 108288. 10.1016/j.isci.2023.108288 Retrieved from https://hdl.handle.net/10161/31493.
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Micah Thomas McClain

Lisa L Satterwhite
Molecular epidemiology of environmental exposures, pesticide exposure and Parkinson’s disease, Cyanobacterial harmful algal blooms and ALS, and creating climate change resilient rural communities.
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2024
2023
https://radx-up.org/grants/rapid-research-pilot/rp2-awardees/
2022
https://dataclimatehealth.duke.edu/teams/
2015
https://www.jyi.org/2015-february/2015/2/1/a-day-on-the-farm-lisa-satterwhites-research-profile

Xiling Shen
Dr. Shen’s research interests lie at precision medicine and systems biology. His lab integrates engineering, computational and biological techniques to study cancer, stem cells, microbiota and the nervous system in the gut. This multidisciplinary work has been instrumental in initiating several translational clinical trials in precision therapy. He is the director of the Woo Center for Big Data and Precision Health (DAP) and a core member of the Center for Genomics and Computational Biology (GCB).

Christopher Wildrick Woods
1. Emerging Infections
2. Global Health
3. Epidemiology of infectious diseases
4. Clinical microbiology and diagnostics
5. Bioterrorism Preparedness
6. Surveillance for communicable diseases
7. Antimicrobial resistance
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