MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management.
| dc.contributor.author | Bellot, Gregory Lucien | |
| dc.contributor.author | Dong, Xiaoke | |
| dc.contributor.author | Lahiri, Amitabha | |
| dc.contributor.author | Sebastin, Sandeep Jacob | |
| dc.contributor.author | Batinic-Haberle, Ines | |
| dc.contributor.author | Pervaiz, Shazib | |
| dc.contributor.author | Puhaindran, Mark Edward | |
| dc.date.accessioned | 2020-07-15T22:19:05Z | |
| dc.date.available | 2020-07-15T22:19:05Z | |
| dc.date.issued | 2019-01 | |
| dc.date.updated | 2020-07-15T22:19:02Z | |
| dc.description.abstract | Negative Pressure Wound Therapy (NPWT), a widely used modality in the management of surgical and trauma wounds, offers clear benefits over conventional wound healing strategies. Despite the wide-ranging effects ascribed to NPWT, the precise molecular mechanisms underlying the accelerated healing supported by NPWT remains poorly understood. Notably, cellular redox status-a product of the balance between cellular reactive oxygen species (ROS) production and anti-oxidant defense systems-plays an important role in wound healing and dysregulation of redox homeostasis has a profound effect on wound healing. Here we investigated potential links between the use of NPWT and the regulation of antioxidant mechanisms. Using patient samples and a rodent model of acute injury, we observed a significant accumulation of MnSOD protein as well as higher enzymatic activity in tissues upon NPWT. As a proof of concept and to outline the important role of SOD activity in wound healing, we replaced NPWT by the topical application of a MnSOD mimetic, Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin (MnTE-2-PyP5+, MnE, BMX-010, AEOl10113) in the rodent model. We observed that MnE is a potent wound healing enhancer as it appears to facilitate the formation of new tissue within the wound bed and consequently advances wound closure by two days, compared to the non-treated animals. Taken together, these results show for the first time a link between NPWT and regulation of antioxidant mechanism through the maintenance of MnSOD activity. Additionally this discovery outlined the potential role of MnSOD mimetics as topical agents enhancing wound healing. | |
| dc.identifier | S2213-2317(18)30524-X | |
| dc.identifier.issn | 2213-2317 | |
| dc.identifier.issn | 2213-2317 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Elsevier BV | |
| dc.relation.ispartof | Redox biology | |
| dc.relation.isversionof | 10.1016/j.redox.2018.10.014 | |
| dc.subject | Animals | |
| dc.subject | Humans | |
| dc.subject | Rats | |
| dc.subject | Disease Models, Animal | |
| dc.subject | Metalloporphyrins | |
| dc.subject | Superoxide Dismutase | |
| dc.subject | Antioxidants | |
| dc.subject | Treatment Outcome | |
| dc.subject | Combined Modality Therapy | |
| dc.subject | Administration, Topical | |
| dc.subject | Biomimetics | |
| dc.subject | Wound Healing | |
| dc.subject | Enzyme Activation | |
| dc.subject | Disease Management | |
| dc.subject | Negative-Pressure Wound Therapy | |
| dc.subject | Biological Mimicry | |
| dc.title | MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management. | |
| dc.type | Journal article | |
| pubs.begin-page | 307 | |
| pubs.end-page | 320 | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Radiation Oncology | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.publication-status | Published | |
| pubs.volume | 20 |
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