Portable, Fiber-Based, Diffuse Reflection Spectroscopy (DRS) Systems for Estimating Tissue Optical Properties.

Vishwanath, Karthik

Chang, Kevin

Klein, Daniel

Deng, Yu Feng

Chang, Vivide

Phelps, Janelle E

Ramanujam, Nimmi

United States

2013-03-06T17:27:22Z

2011-02-01

Steady-state diffuse reflection spectroscopy is a well-studied optical technique that can provide a noninvasive and quantitative method for characterizing the absorption and scattering properties of biological tissues. Here, we compare three fiber-based diffuse reflection spectroscopy systems that were assembled to create a light-weight, portable, and robust optical spectrometer that could be easily translated for repeated and reliable use in mobile settings. The three systems were built using a broadband light source and a compact, commercially available spectrograph. We tested two different light sources and two spectrographs (manufactured by two different vendors). The assembled systems were characterized by their signal-to-noise ratios, the source-intensity drifts, and detector linearity. We quantified the performance of these instruments in extracting optical properties from diffuse reflectance spectra in tissue-mimicking liquid phantoms with well-controlled optical absorption and scattering coefficients. We show that all assembled systems were able to extract the optical absorption and scattering properties with errors less than 10%, while providing greater than ten-fold decrease in footprint and cost (relative to a previously well-characterized and widely used commercial system). Finally, we demonstrate the use of these small systems to measure optical biomarkers in vivo in a small-animal model cancer therapy study. We show that optical measurements from the simple portable system provide estimates of tumor oxygen saturation similar to those detected using the commercial system in murine tumor models of head and neck cancer.

http://www.ncbi.nlm.nih.gov/pubmed/21499501

1943-3530

https://hdl.handle.net/10161/6344

eng

SAGE Publications

Appl Spectrosc

10.1366/10-06052

Applied Spectroscopy

Portable, Fiber-Based, Diffuse Reflection Spectroscopy (DRS) Systems for Estimating Tissue Optical Properties.

Journal article

2

65

http://www.ncbi.nlm.nih.gov/pubmed/21499501

206

215

2

Basic Science Departments

Biomedical Engineering

Duke

Duke Cancer Institute

Duke Science & Society

Global Health Institute

Initiatives

Institutes and Centers

Institutes and Provost's Academic Units

Pharmacology & Cancer Biology

Pratt School of Engineering

School of Medicine

University Institutes and Centers

Published

62