The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.

Walton, Eric M; Cronan, Mark R; Beerman, Rebecca W; Tobin, David M

The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.

dc.contributor.author	Walton, Eric M
dc.contributor.author	Cronan, Mark R
dc.contributor.author	Beerman, Rebecca W
dc.contributor.author	Tobin, David M
dc.contributor.editor	Gong, Zhiyuan
dc.coverage.spatial	United States
dc.date.accessioned	2015-11-10T19:02:55Z
dc.date.issued	2015
dc.description.abstract	Transgenic labeling of innate immune cell lineages within the larval zebrafish allows for real-time, in vivo analyses of microbial pathogenesis within a vertebrate host. To date, labeling of zebrafish macrophages has been relatively limited, with the most specific expression coming from the mpeg1 promoter. However, mpeg1 transcription at both endogenous and transgenic loci becomes attenuated in the presence of intracellular pathogens, including Salmonella typhimurium and Mycobacterium marinum. Here, we describe mfap4 as a macrophage-specific promoter capable of producing transgenic lines in which transgene expression within larval macrophages remains stable throughout several days of infection. Additionally, we have developed a novel macrophage-specific Cre transgenic line under the control of mfap4, enabling macrophage-specific expression using existing floxed transgenic lines. These tools enrich the repertoire of transgenic lines and promoters available for studying zebrafish macrophage dynamics during infection and inflammation and add flexibility to the design of future macrophage-specific transgenic lines.
dc.identifier	http://www.ncbi.nlm.nih.gov/pubmed/26445458
dc.identifier	PONE-D-15-31888
dc.identifier.eissn	1932-6203
dc.identifier.uri	https://hdl.handle.net/10161/10845
dc.language	eng
dc.publisher	Public Library of Science (PLoS)
dc.relation.ispartof	PLoS One
dc.relation.isversionof	10.1371/journal.pone.0138949
dc.subject	Animals
dc.subject	Animals, Genetically Modified
dc.subject	Cell Lineage
dc.subject	Disease Models, Animal
dc.subject	Host-Pathogen Interactions
dc.subject	Immunity, Innate
dc.subject	Larva
dc.subject	Macrophages
dc.subject	Mycobacterium Infections
dc.subject	Mycobacterium marinum
dc.subject	Promoter Regions, Genetic
dc.subject	Salmonella typhimurium
dc.subject	Transgenes
dc.subject	Zebrafish
dc.subject	Zebrafish Proteins
dc.title	The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.
dc.type	Journal article
duke.contributor.orcid	Beerman, Rebecca W\|0000-0002-2057-4170
duke.contributor.orcid	Tobin, David M\|0000-0003-3465-5518
pubs.author-url	http://www.ncbi.nlm.nih.gov/pubmed/26445458
pubs.begin-page	e0138949
pubs.issue	10
pubs.organisational-group	Basic Science Departments
pubs.organisational-group	Duke
pubs.organisational-group	Immunology
pubs.organisational-group	Molecular Genetics and Microbiology
pubs.organisational-group	School of Medicine
pubs.publication-status	Published online
pubs.volume	10

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