Host range, transmissibility and antigenicity of a pangolin coronavirus.

Hou, Yixuan J

Chiba, Shiho

Leist, Sarah R

Meganck, Rita M

Martinez, David R

Schäfer, Alexandra

Catanzaro, Nicholas J

Sontake, Vishwaraj

West, Ande

Edwards, Catlin E

Yount, Boyd

Lee, Rhianna E

Gallant, Samuel C

Zost, Seth J

Powers, John

Adams, Lily

Kong, Edgar F

Mattocks, Melissa

Tata, Aleksandra

Randell, Scott H

Tata, Purushothama R

Halfmann, Peter

Crowe, James E

Kawaoka, Yoshihiro

Baric, Ralph S

2024-02-01T17:56:54Z

2024-02-01T17:56:54Z

2023-10

The pathogenic and cross-species transmission potential of SARS-CoV-2-related coronaviruses (CoVs) remain poorly characterized. Here we recovered a wild-type pangolin (Pg) CoV GD strain including derivatives encoding reporter genes using reverse genetics. In primary human cells, PgCoV replicated efficiently but with reduced fitness and showed less efficient transmission via airborne route compared with SARS-CoV-2 in hamsters. PgCoV was potently inhibited by US Food and Drug Administration approved drugs, and neutralized by COVID-19 patient sera and SARS-CoV-2 therapeutic antibodies in vitro. A pan-Sarbecovirus antibody and SARS-CoV-2 S2P recombinant protein vaccine protected BALB/c mice from PgCoV infection. In K18-hACE2 mice, PgCoV infection caused severe clinical disease, but mice were protected by a SARS-CoV-2 human antibody. Efficient PgCoV replication in primary human cells and hACE2 mice, coupled with a capacity for airborne spread, highlights an emergence potential. However, low competitive fitness, pre-immune humans and the benefit of COVID-19 countermeasures should impede its ability to spread globally in human populations.

10.1038/s41564-023-01476-x

2058-5276

2058-5276

https://hdl.handle.net/10161/30078

eng

Springer Science and Business Media LLC

Nature microbiology

10.1038/s41564-023-01476-x

https://creativecommons.org/licenses/by-nc/4.0

Animals

Mice, Inbred BALB C

Humans

Mice

Antibodies, Viral

Cricetinae

Host Specificity

COVID-19

SARS-CoV-2

COVID-19 Vaccines

Pangolins

Severe acute respiratory syndrome-related coronavirus

Host range, transmissibility and antigenicity of a pangolin coronavirus.

Journal article

Tata, Aleksandra|0000-0003-3270-0485

Tata, Purushothama R|0000-0003-4837-0337

1820

1833

10

Duke

School of Medicine

Basic Science Departments

Clinical Science Departments

Institutes and Centers

Cell Biology

Medicine

Medicine, Pulmonary, Allergy, and Critical Care Medicine

Duke Cancer Institute

Regeneration Next Initiative

Published

8