Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.
| dc.contributor.author | Shaked, Natan T | |
| dc.contributor.author | Satterwhite, Lisa L | |
| dc.contributor.author | Bursac, Nenad | |
| dc.contributor.author | Wax, Adam | |
| dc.coverage.spatial | United States | |
| dc.date.accessioned | 2014-04-16T17:10:23Z | |
| dc.date.issued | 2010-08-23 | |
| dc.description.abstract | We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution. The whole-cell phase profiles are analyzed to yield valuable quantitative parameters characterizing the cell dynamics, without the need to decouple thickness from refractive index differences. Our experimental results verify that these new parameters can be used with wide field interferometric microscopy to discriminate the modulation of cardiomyocyte contraction dynamics due to temperature variation. To demonstrate the necessity of the proposed numerical analysis for cardiomyocytes, we present confocal dual-fluorescence-channel microscopy results which show that the rapid motion of the cell organelles during contraction preclude assuming a homogenous refractive index over the entire cell contents, or using multiple-exposure or scanning microscopy. | |
| dc.identifier | ||
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Optica Publishing Group | |
| dc.relation.ispartof | Biomed Opt Express | |
| dc.relation.isversionof | 10.1364/BOE.1.000706 | |
| dc.title | Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy. | |
| dc.type | Journal article | |
| duke.contributor.orcid | Bursac, Nenad|0000-0002-5688-6061 | |
| pubs.author-url | ||
| pubs.begin-page | 706 | |
| pubs.end-page | 719 | |
| pubs.issue | 2 | |
| pubs.organisational-group | Biomedical Engineering | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | Duke Cancer Institute | |
| pubs.organisational-group | Duke Institute for Brain Sciences | |
| pubs.organisational-group | Institutes and Centers | |
| pubs.organisational-group | Institutes and Provost's Academic Units | |
| pubs.organisational-group | Medicine | |
| pubs.organisational-group | Medicine, Cardiology | |
| pubs.organisational-group | Pratt School of Engineering | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | University Institutes and Centers | |
| pubs.publication-status | Published online | |
| pubs.volume | 1 |
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