Placental Expression of ACE2 and TMPRSS2 in Maternal Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Are Placental Defenses Mediated by Fetal Sex?
| dc.contributor.author | Shook, Lydia L | |
| dc.contributor.author | Bordt, Evan A | |
| dc.contributor.author | Meinsohn, Marie-Charlotte | |
| dc.contributor.author | Pepin, David | |
| dc.contributor.author | De Guzman, Rose M | |
| dc.contributor.author | Brigida, Sara | |
| dc.contributor.author | Yockey, Laura J | |
| dc.contributor.author | James, Kaitlyn E | |
| dc.contributor.author | Sullivan, Mackenzie W | |
| dc.contributor.author | Bebell, Lisa M | |
| dc.contributor.author | Roberts, Drucilla J | |
| dc.contributor.author | Kaimal, Anjali J | |
| dc.contributor.author | Li, Jonathan Z | |
| dc.contributor.author | Schust, Danny | |
| dc.contributor.author | Gray, Kathryn J | |
| dc.contributor.author | Edlow, Andrea G | |
| dc.date.accessioned | 2023-06-12T17:23:04Z | |
| dc.date.available | 2023-06-12T17:23:04Z | |
| dc.date.issued | 2021-12 | |
| dc.date.updated | 2023-06-12T17:23:03Z | |
| dc.description.abstract | BackgroundExpression of angiotensin-converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2), host molecules required for viral entry, may underlie sex differences in vulnerability to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We investigated whether placental ACE2 and TMPRSS2 expression vary by fetal sex in the presence of maternal SARS-CoV-2 infection.MethodsPlacental ACE2 and TMPRSS2 expression was quantified by quantitative reverse transcription polymerase chain reaction (RT-PCR) and by Western blot in 68 pregnant women (38 SARS-CoV-2 positive, 30 SARS-CoV-2 negative) delivering at Mass General Brigham from April to June 2020. The impact of fetal sex and maternal SARS-CoV-2 exposure on ACE2 and TMPRSS2 was analyzed by 2-way analysis of variance (ANOVA).ResultsMaternal SARS-CoV-2 infection impacted placental TMPRSS2 expression in a sexually dimorphic fashion (2-way ANOVA interaction, P = .002). We observed no impact of fetal sex or maternal SARS-CoV-2 status on ACE2. TMPRSS2 expression was significantly correlated with ACE2 expression in males (Spearman ρ = 0.54, P = .02) but not females (ρ = 0.23, P = .34) exposed to maternal SARS-CoV-2.ConclusionsSex differences in placental TMPRSS2 but not ACE2 were observed in the setting of maternal SARS-CoV-2 infection, which may have implications for offspring vulnerability to placental infection. | |
| dc.identifier | 6325592 | |
| dc.identifier.issn | 0022-1899 | |
| dc.identifier.issn | 1537-6613 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Oxford University Press (OUP) | |
| dc.relation.ispartof | The Journal of infectious diseases | |
| dc.relation.isversionof | 10.1093/infdis/jiab335 | |
| dc.subject | Fetal Blood | |
| dc.subject | Fetus | |
| dc.subject | Placenta | |
| dc.subject | Humans | |
| dc.subject | Pregnancy Complications, Infectious | |
| dc.subject | Serine Endopeptidases | |
| dc.subject | Sex Factors | |
| dc.subject | Gene Expression | |
| dc.subject | Pregnancy | |
| dc.subject | Adult | |
| dc.subject | Female | |
| dc.subject | Male | |
| dc.subject | Infectious Disease Transmission, Vertical | |
| dc.subject | COVID-19 | |
| dc.subject | Angiotensin-Converting Enzyme 2 | |
| dc.subject | SARS-CoV-2 | |
| dc.title | Placental Expression of ACE2 and TMPRSS2 in Maternal Severe Acute Respiratory Syndrome Coronavirus 2 Infection: Are Placental Defenses Mediated by Fetal Sex? | |
| dc.type | Journal article | |
| duke.contributor.orcid | Schust, Danny|0000-0003-4561-7808 | |
| pubs.begin-page | S647 | |
| pubs.end-page | S659 | |
| pubs.issue | Suppl 6 | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Obstetrics and Gynecology | |
| pubs.organisational-group | Obstetrics and Gynecology, Reproductive Endocrinology & Fertility | |
| pubs.publication-status | Published | |
| pubs.volume | 224 |
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