Targeted repair of heart injury by stem cells fused with platelet nanovesicles.

Tang, Junnan

Su, Teng

Huang, Ke

Dinh, Phuong-Uyen

Wang, Zegen

Vandergriff, Adam

Hensley, Michael T

Cores, Jhon

Allen, Tyler

Li, Taosheng

Sproul, Erin

Mihalko, Emily

Lobo, Leonard J

Ruterbories, Laura

Lynch, Alex

Brown, Ashley

Caranasos, Thomas G

Shen, Deliang

Stouffer, George A

Gu, Zhen

Zhang, Jinying

Cheng, Ke

2022-12-04T18:15:10Z

2022-12-04T18:15:10Z

2018-01

2022-12-04T18:15:07Z

Stem cell transplantation, as used clinically, suffers from low retention and engraftment of the transplanted cells. Inspired by the ability of platelets to recruit stem cells to sites of injury on blood vessels, we hypothesized that platelets might enhance the vascular delivery of cardiac stem cells (CSCs) to sites of myocardial infarction injury. Here, we show that CSCs with platelet nanovesicles fused onto their surface membranes express platelet surface markers that are associated with platelet adhesion to injury sites. We also find that the modified CSCs selectively bind collagen-coated surfaces and endothelium-denuded rat aortas, and that in rat and porcine models of acute myocardial infarction the modified CSCs increase retention in the heart and reduce infarct size. Platelet-nanovesicle-fused CSCs thus possess the natural targeting and repairing ability of their parental cell types. This stem cell manipulation approach is fast, straightforward and safe, does not require genetic alteration of the cells, and should be generalizable to multiple cell types.

10.1038/s41551-017-0182-x

2157-846X

2157-846X

https://hdl.handle.net/10161/26328

eng

Springer Science and Business Media LLC

Nature biomedical engineering

10.1038/s41551-017-0182-x

Targeted repair of heart injury by stem cells fused with platelet nanovesicles.

Journal article

Su, Teng|0000-0001-7888-0763

Allen, Tyler|0000-0002-8729-6339

17

26

1

Duke

School of Medicine

Clinical Science Departments

Medicine

Medicine, Cardiology

Published

2