SECTM1 Produced by Tumor Cells Attracts Human Monocytes Via CD7-mediated Activation of the PI3K Pathways
| dc.contributor.author | Kaufman, RE | |
| dc.date.accessioned | 2023-02-01T14:15:18Z | |
| dc.date.available | 2023-02-01T14:15:18Z | |
| dc.date.issued | 2013-11-13 | |
| dc.date.updated | 2023-02-01T14:15:16Z | |
| dc.description.abstract | Tumor-associated macrophages (TAMs) have essential roles in tumor progression and metastasis. Tumor cells recruit myeloid progenitors and monocytes to the tumor site, where they differentiate into TAMs; however, this process is not well studied in humans. Here we show that human CD7, a T-cell and NK cell receptor, is highly expressed by monocytes and macrophages. Expression of CD7 decreases in M-CSF-differentiated macrophages and in melanoma-conditioned medium-induced macrophages (MCMI/Mφ) in comparison to monocytes. A ligand for CD7, SECTM1 (secreted and transmembrane protein 1), is highly expressed in many tumors, including melanoma cells. We show that SECTM1 binds to CD7 and significantly increases monocyte migration by activation of the PI3K (phosphatidylinositol 3'-kinase) pathway. In human melanoma tissues, tumor-infiltrating macrophages expressing CD7 are present. These melanomas, with CD7-positive inflammatory cell infiltrations, frequently highly express SECTM1, including an N-terminal, soluble form, which can be detected in the sera of metastatic melanoma patients but not in normal sera. Taken together, our data demonstrate that CD7 is present on monocytes and tumor macrophages and that its ligand, SECTM1, is frequently expressed in corresponding melanoma tissues, possibly acting as a chemoattractant for monocytes to modulate the melanoma microenvironment. | |
| dc.identifier | S0022-202X(15)36719-1 | |
| dc.identifier.issn | 0022-202X | |
| dc.identifier.issn | 1523-1747 | |
| dc.identifier.uri | ||
| dc.language | eng | |
| dc.publisher | Elsevier BV | |
| dc.relation.ispartof | J. Investigative Dermatology | |
| dc.relation.isversionof | 10.1038/jid.2013.437 | |
| dc.subject | Monocytes | |
| dc.subject | Macrophages | |
| dc.subject | Melanocytes | |
| dc.subject | Humans | |
| dc.subject | Melanoma | |
| dc.subject | Neoplasm Metastasis | |
| dc.subject | Disease Progression | |
| dc.subject | Macrophage Colony-Stimulating Factor | |
| dc.subject | Interferon-alpha | |
| dc.subject | Membrane Proteins | |
| dc.subject | Antigens, CD7 | |
| dc.subject | Chemotactic Factors | |
| dc.subject | Culture Media, Conditioned | |
| dc.subject | Ligands | |
| dc.subject | Signal Transduction | |
| dc.subject | Apoptosis | |
| dc.subject | Cell Differentiation | |
| dc.subject | Cell Proliferation | |
| dc.subject | Cell Movement | |
| dc.subject | Gene Expression Regulation, Neoplastic | |
| dc.subject | Protein Structure, Tertiary | |
| dc.subject | Phosphatidylinositol 3-Kinases | |
| dc.subject | Proteasome Inhibitors | |
| dc.title | SECTM1 Produced by Tumor Cells Attracts Human Monocytes Via CD7-mediated Activation of the PI3K Pathways | |
| dc.type | Journal article | |
| duke.contributor.orcid | Kaufman, RE|0000-0003-4612-4861 | |
| pubs.begin-page | 1108 | |
| pubs.end-page | 1118 | |
| pubs.issue | 4 | |
| pubs.organisational-group | Duke | |
| pubs.organisational-group | School of Medicine | |
| pubs.organisational-group | Clinical Science Departments | |
| pubs.organisational-group | Medicine | |
| pubs.organisational-group | Medicine, Medical Oncology | |
| pubs.publication-status | Published | |
| pubs.volume | 134 |
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