Caloric restriction alters the metabolic response to a mixed-meal: results from a randomized, controlled trial.

Huffman, Kim M

Redman, Leanne M

Landerman, Lawrence R

Pieper, Carl F

Stevens, Robert D

Muehlbauer, Michael J

Wenner, Brett R

Bain, James R

Kraus, Virginia B

Newgard, Christopher B

Ravussin, Eric

Kraus, William E

Tomé, Daniel

United States

2015-11-10T22:38:43Z

2012

OBJECTIVES: To determine if caloric restriction (CR) would cause changes in plasma metabolic intermediates in response to a mixed meal, suggestive of changes in the capacity to adapt fuel oxidation to fuel availability or metabolic flexibility, and to determine how any such changes relate to insulin sensitivity (S(I)). METHODS: Forty-six volunteers were randomized to a weight maintenance diet (Control), 25% CR, or 12.5% CR plus 12.5% energy deficit from structured aerobic exercise (CR+EX), or a liquid calorie diet (890 kcal/d until 15% reduction in body weight)for six months. Fasting and postprandial plasma samples were obtained at baseline, three, and six months. A targeted mass spectrometry-based platform was used to measure concentrations of individual free fatty acids (FFA), amino acids (AA), and acylcarnitines (AC). S(I) was measured with an intravenous glucose tolerance test. RESULTS: Over three and six months, there were significantly larger differences in fasting-to-postprandial (FPP) concentrations of medium and long chain AC (byproducts of FA oxidation) in the CR relative to Control and a tendency for the same in CR+EX (CR-3 month P = 0.02; CR-6 month P = 0.002; CR+EX-3 month P = 0.09; CR+EX-6 month P = 0.08). After three months of CR, there was a trend towards a larger difference in FPP FFA concentrations (P = 0.07; CR-3 month P = 0.08). Time-varying differences in FPP concentrations of AC and AA were independently related to time-varying S(I) (P<0.05 for both). CONCLUSIONS: Based on changes in intermediates of FA oxidation following a food challenge, CR imparted improvements in metabolic flexibility that correlated with improvements in S(I). TRIAL REGISTRATION: ClinicalTrials.gov NCT00099151.

http://www.ncbi.nlm.nih.gov/pubmed/22523532

PONE-D-11-13098

1932-6203

https://hdl.handle.net/10161/10872

eng

Public Library of Science (PLoS)

PLoS One

10.1371/journal.pone.0028190

Adult

Amino Acids

Caloric Restriction

Carnitine

Energy Intake

Exercise

Fasting

Fatty Acids, Nonesterified

Female

Glucose Tolerance Test

Humans

Insulin Resistance

Male

Middle Aged

Overweight

Postprandial Period

Principal Component Analysis

Caloric restriction alters the metabolic response to a mixed-meal: results from a randomized, controlled trial.

Journal article

Pieper, Carl F|0000-0003-4809-1725

Bain, James R|0000-0002-8917-9187

Kraus, Virginia B|0000-0001-8173-8258

Kraus, William E|0000-0003-1930-9684

http://www.ncbi.nlm.nih.gov/pubmed/22523532

e28190

4

Basic Science Departments

Biochemistry

Biomedical Engineering

Biostatistics & Bioinformatics

Center for the Study of Aging and Human Development

Clinical Science Departments

Duke

Duke Cancer Institute

Duke Molecular Physiology Institute

Global Health Institute

Institutes and Centers

Institutes and Provost's Academic Units

Medicine

Medicine, Cardiology

Medicine, Endocrinology, Metabolism, and Nutrition

Medicine, Geriatrics

Medicine, Rheumatology and Immunology

Orthopaedics

Pathology

Pharmacology & Cancer Biology

Pratt School of Engineering

Sarah Stedman Nutrition & Metabolism Center

School of Medicine

School of Nursing

School of Nursing - Secondary Group

University Institutes and Centers

Published

7