Heterogenous biofilm mass-transport model replicates periphery sequestration of antibiotics in <i>Pseudomonas aeruginosa</i> PAO1 microcolonies
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2023-11-21
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<jats:p> A model for antibiotic accumulation in bacterial biofilm microcolonies utilizing heterogenous porosity and attachment site profiles replicated the periphery sequestration reported in prior experimental studies on <jats:italic>Pseudomonas aeruginosa PAO1</jats:italic> biofilm cell clusters. These <jats:italic>P. aeruginosa</jats:italic> cell clusters are in vitro models of the chronic <jats:italic>P. aeruginosa</jats:italic> infections in cystic fibrosis patients which display recalcitrance to antibiotic treatments, leading to exacerbated morbidity and mortality. This resistance has been partially attributed to periphery sequestration, where antibiotics fail to penetrate biofilm cell clusters. The physical phenomena driving this periphery sequestration have not been definitively established. This paper introduces mathematical models to account for two proposed physical phenomena driving periphery sequestration: biofilm matrix attachment and volume-exclusion due to variable biofilm porosity. An antibiotic accumulation model which incorporated these phenomena better fit observed periphery sequestration data compared to previous models. </jats:p>
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Prince, Joshua, and A-Andrew D Jones (2023). Heterogenous biofilm mass-transport model replicates periphery sequestration of antibiotics in Pseudomonas aeruginosa PAO1 microcolonies. Proceedings of the National Academy of Sciences, 120(47). 10.1073/pnas.2312995120 Retrieved from https://hdl.handle.net/10161/29401.
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A-Andrew D. Jones
Dr. Jones' research uses engineering and policy analysis to help solve global challenges related to water and health. He is a 2021 recipient of the NIH R35 Maximizing Investigator’s Research Award to develop new models and tools for studying biofilms and a 2019 Sloan SEED fund award to develop new tools for point of use water quality monitoring systems. He was recognized by a Young Investigator Award from the Center for Biofilm Engineering at Montana State, the premier center for biofilm research in the US. He received a BS in Mathematics and BS, MS, and PhD in Mechanical Engineering from MIT where he was a Lemelson Presidential Fellow and Alfred P. Sloan UCEM Scholar. He completed post-doctoral training as a Future Faculty Fellow at Northeastern University. He has directly supervised 2 high school students, over 20 undergraduates, 5 MS, 5 PhD, and 2 post-doctoral trainees including 8 from underrepresented backgrounds and 19 women. He and his team have presented at over 60 conferences and seminars. He served as a Pratt Coaching 360 advisor and Duke UCEM Faculty Champion for Civil & Environmental Engineering.
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