Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice.

dc.contributor.author

Periera-Simon, Simone

dc.contributor.author

Xia, Xiaomei

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Catanuto, Paola

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Coronado, Ramon

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Kurtzberg, Joanne

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Bellio, Michael

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Lee, Yee-Shuan

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Khan, Aisha

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Smith, Robin

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Elliot, Sharon J

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Glassberg, Marilyn K

dc.date.accessioned

2022-03-25T20:08:45Z

dc.date.available

2022-03-25T20:08:45Z

dc.date.issued

2021-02

dc.date.updated

2022-03-25T20:08:44Z

dc.description.abstract

Background and objective

IPF is a fatal and debilitating lung disorder increasing in incidence worldwide. To date, two approved treatments only slow disease progression, have multiple side effects and do not provide a cure. MSC have promising therapeutic potential as a cell-based therapy for many lung disorders based on the anti-fibrotic properties of the MSC.

Methods

Critical questions remain surrounding the optimal source, timing and efficacy of cell-based therapies. The present study examines the most effective sources of MSC. Human MSC were derived from adipose, WJ, chorionic membrane (CSC) and chorionic villi (CVC). MSC were injected into the ageing mouse model of BLM-induced lung fibrosis.

Results

All sources decreased Aschroft and hydroxyproline levels when injected into BLM-treated mice at day 10 with the exception of CSC cells that did not change hydroxyproline levels. There were also decreases in mRNA expression of αv -integrin and TNFα in all sources except CSC. Only ASC- and WJ-derived cells reduced AKT and MMP-2 activation, while Cav-1 was increased by ASC treatment as previously reported. BLM-induced miR dysregulation of miR-29 and miR-199 was restored only by ASC treatment.

Conclusion

Our data suggest that sources of MSC may differ in the pathway(s) involved in repair.
dc.identifier.issn

1323-7799

dc.identifier.issn

1440-1843

dc.identifier.uri

https://hdl.handle.net/10161/24724

dc.language

eng

dc.publisher

Wiley

dc.relation.ispartof

Respirology (Carlton, Vic.)

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10.1111/resp.13928

dc.subject

Mesenchymal Stem Cells

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Animals

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Mice, Inbred C57BL

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Humans

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Pulmonary Fibrosis

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Disease Models, Animal

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Inflammation

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Bleomycin

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MicroRNAs

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RNA, Messenger

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Mesenchymal Stem Cell Transplantation

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Transplantation, Homologous

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Gene Expression Regulation

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Adult

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Male

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Proto-Oncogene Proteins c-akt

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Caveolin 1

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Matrix Metalloproteinase 2

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Biomarkers

dc.title

Anti-fibrotic effects of different sources of MSC in bleomycin-induced lung fibrosis in C57BL6 male mice.

dc.type

Journal article

duke.contributor.orcid

Kurtzberg, Joanne|0000-0002-3370-0703

pubs.begin-page

161

pubs.end-page

170

pubs.issue

2

pubs.organisational-group

Duke

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School of Medicine

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Clinical Science Departments

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Institutes and Centers

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Pathology

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Pediatrics

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Duke Cancer Institute

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Institutes and Provost's Academic Units

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Initiatives

pubs.organisational-group

Duke Innovation & Entrepreneurship

pubs.organisational-group

Pediatrics, Transplant and Cellular Therapy

pubs.publication-status

Published

pubs.volume

26

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