Characterization of the Foreign Body Response to Common Surgical Biomaterials in a Murine Model.

Ibrahim, Mohamed

Bond, Jennifer

Medina, Manuel A

Chen, Lei

Quiles, Carlos

Kokosis, George

Bashirov, Latif

Klitzman, Bruce

Levinson, Howard

2019-05-01T13:51:01Z

2019-05-01T13:51:01Z

2017-11

2019-05-01T13:51:00Z

BACKGROUND:Implanted biomaterials are subject to a significant reaction from the host, known as the foreign body response (FBR). We quantified the FBR to five materials following subcutaneous implantation in mice. MATERIALS AND METHODS:Polyvinyl alcohol (PVA) and silicone sheets are considered highly biocompatible biomaterials and were cut into 8mm-diameter disks. Expanded PTFE (ePTFE)and polypropylene are also widely used biocompatible biomaterials and were cut into 2cm-long cylinders. Cotton was selected as a negative control material that would invoke an intense FBR, was cut into disks and implanted. The implants were inserted subcutaneously into female C57BL/6 mice. On post-implantation days 14, 30, 60, 90 and 180, implants were retrieved. Cellularity was assessed with DAPI stain, collagen with Masson's trichrome stain. mast cells with toluidine-blue, macrophages with F4/80 immunohistochemical-stain, and capsular thickness and foreign body giant cells with hematoxylin & eosin. RESULTS:DAPI revealed a significantly increased cellularity in both PVA andsilicone, and ePTFE had the lowest cell density. Silicone showed the lowest cellularity at d14 and d90 whereas ePTFE showed the lowest cellularity at days 30, 60, and 180. Masson's trichrome staining demonstrated no apparent difference in collagen. Toluidine blue showed no differences in mast cells. There were, however, fewer macrophages associated with ePTFE. On d14, PVA had highest number of macrophages, whereas polypropylene had the highest number at all time points after d14. Giant cells increased earlier and gradually decreased later. On d90, PVA exhibited a significantly increased number of giant cells compared to polypropylene and silicone. Silicone consistently formed the thinnest capsule throughout all time points. On d14, cotton had formed the thickest capsule. On d30 polypropylenehas formed thickest capsule and on days 60, 90 and 180, PVA had formed thickest capsule. CONCLUSION:These data reveal differences in capsule thickness and cellular response in an implant-related manor, indicating that fibrotic reactions to biomaterials are implant specific and should be carefully considered when performing studies on fibrosis when biomaterials are being used.

0930-343X

1435-0130

https://hdl.handle.net/10161/18478

eng

Springer Science and Business Media LLC

European journal of plastic surgery

10.1007/s00238-017-1308-9

PVA

Prolene

biocompatibility

ePTFE

fibrosis

implants

polypropylene

Characterization of the Foreign Body Response to Common Surgical Biomaterials in a Murine Model.

Journal article

383

392

5

School of Medicine

Duke

Biomedical Engineering

Pratt School of Engineering

Cell Biology

Basic Science Departments

Duke Innovation & Entrepreneurship

Initiatives

Institutes and Provost's Academic Units

Surgery, Plastic, Maxillofacial, and Oral Surgery

Surgery

Clinical Science Departments

Dermatology

Pathology

Published

40