Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response.

dc.contributor.author

McBroom, Amanda J

dc.contributor.author

Kuehn, Meta J

dc.coverage.spatial

England

dc.date.accessioned

2015-10-05T18:42:34Z

dc.date.issued

2007-01

dc.description.abstract

Conditions that impair protein folding in the Gram-negative bacterial envelope cause stress. The destabilizing effects of stress in this compartment are recognized and countered by a number of signal transduction mechanisms. Data presented here reveal another facet of the complex bacterial stress response, release of outer membrane vesicles. Native vesicles are composed of outer membrane and periplasmic material, and they are released from the bacterial surface without loss of membrane integrity. Here we demonstrate that the quantity of vesicle release correlates directly with the level of protein accumulation in the cell envelope. Accumulation of material occurs under stress, and is exacerbated upon impairment of the normal housekeeping and stress-responsive mechanisms of the cell. Mutations that cause increased vesiculation enhance bacterial survival upon challenge with stressing agents or accumulation of toxic misfolded proteins. Preferential packaging of a misfolded protein mimic into vesicles for removal indicates that the vesiculation process can act to selectively eliminate unwanted material. Our results demonstrate that production of bacterial outer membrane vesicles is a fully independent, general envelope stress response. In addition to identifying a novel mechanism for alleviating stress, this work provides physiological relevance for vesicle production as a protective mechanism.

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/17163978

dc.identifier

MMI5522

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0950-382X

dc.identifier.uri

https://hdl.handle.net/10161/10658

dc.language

eng

dc.publisher

Wiley

dc.relation.ispartof

Mol Microbiol

dc.relation.isversionof

10.1111/j.1365-2958.2006.05522.x

dc.subject

Adaptation, Physiological

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Anti-Bacterial Agents

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Bacterial Outer Membrane Proteins

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Cell Wall

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DNA Transposable Elements

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Electrophoresis, Polyacrylamide Gel

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Escherichia coli

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Escherichia coli Proteins

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Gram-Negative Bacteria

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Microbial Viability

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Microscopy, Electron, Scanning

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Microscopy, Electron, Transmission

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Mutagenesis, Insertional

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Polymyxin B

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Protein Transport

dc.title

Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response.

dc.type

Journal article

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/17163978

pubs.begin-page

545

pubs.end-page

558

pubs.issue

2

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Biochemistry

pubs.organisational-group

Duke

pubs.organisational-group

Duke Cancer Institute

pubs.organisational-group

Institutes and Centers

pubs.organisational-group

Molecular Genetics and Microbiology

pubs.organisational-group

School of Medicine

pubs.publication-status

Published

pubs.volume

63

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