Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke.

Li, Xuan; Lyu, Jingjun; Li, Ran; Jain, Vaibhav; Shen, Yuntian; Del Águila, Ángela; Hoffmann, Ulrike; Sheng, Huaxin; Yang, Wei

Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke.

dc.contributor.author	Li, Xuan
dc.contributor.author	Lyu, Jingjun
dc.contributor.author	Li, Ran
dc.contributor.author	Jain, Vaibhav
dc.contributor.author	Shen, Yuntian
dc.contributor.author	Del Águila, Ángela
dc.contributor.author	Hoffmann, Ulrike
dc.contributor.author	Sheng, Huaxin
dc.contributor.author	Yang, Wei
dc.date.accessioned	2022-04-21T16:00:05Z
dc.date.available	2022-04-21T16:00:05Z
dc.date.issued	2022-04-07
dc.date.updated	2022-04-21T16:00:05Z
dc.description.abstract	Background Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. Methods Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. Results We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. Conclusions This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.
dc.identifier	10.1186/s12974-022-02447-5
dc.identifier.issn	1742-2094
dc.identifier.issn	1742-2094
dc.identifier.uri	https://hdl.handle.net/10161/24851
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	Journal of neuroinflammation
dc.relation.isversionof	10.1186/s12974-022-02447-5
dc.subject	Brain
dc.subject	Microglia
dc.subject	Animals
dc.subject	Mice
dc.subject	Stroke
dc.subject	Single-Cell Analysis
dc.subject	Transcriptome
dc.subject	Ischemic Stroke
dc.title	Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke.
dc.type	Journal article
duke.contributor.orcid	Sheng, Huaxin\|0000-0002-4325-2940
duke.contributor.orcid	Yang, Wei\|0000-0001-5719-4393
pubs.begin-page	83
pubs.issue	1
pubs.organisational-group	Duke
pubs.organisational-group	School of Medicine
pubs.organisational-group	Clinical Science Departments
pubs.organisational-group	Anesthesiology
pubs.publication-status	Published
pubs.volume	19