Curved FtsZ protofilaments generate bending forces on liposome membranes.

dc.contributor.author

Osawa, Masaki

dc.contributor.author

Anderson, David E

dc.contributor.author

Erickson, Harold P

dc.date.accessioned

2021-02-01T17:05:54Z

dc.date.available

2021-02-01T17:05:54Z

dc.date.issued

2009-11

dc.date.updated

2021-02-01T17:05:49Z

dc.description.abstract

We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction force. When added to the outside of liposomes, FtsZ-YFP-mts bound and produced concave depressions, bending the membrane in the same direction as the Z ring inside liposomes. Prominent membrane tubules were then extruded at the intersections of concave depressions. We tested the effect of moving the membrane-targeting sequence (mts) from the C-terminus to the N-terminus, which is approximately 180 degrees from the C-terminal tether. When mts-FtsZ-YFP was applied to the outside of liposomes, it generated convex bulges, bending the membrane in the direction opposite to the concave depressions. We conclude that FtsZ protofilaments have a fixed direction of curvature, and the direction of membrane bending depends on which side of the bent protofilament the mts is attached to. This supports models in which the FtsZ constriction force is generated by protofilament bending.

dc.identifier

emboj2009277

dc.identifier.issn

0261-4189

dc.identifier.issn

1460-2075

dc.identifier.uri

https://hdl.handle.net/10161/22300

dc.language

eng

dc.publisher

Wiley

dc.relation.ispartof

The EMBO journal

dc.relation.isversionof

10.1038/emboj.2009.277

dc.subject

Membranes

dc.subject

Cytoskeleton

dc.subject

Bacterial Proteins

dc.subject

Cytoskeletal Proteins

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Liposomes

dc.subject

Protein Structure, Quaternary

dc.subject

Structure-Activity Relationship

dc.subject

Tensile Strength

dc.subject

Models, Biological

dc.subject

Models, Molecular

dc.subject

Torsion, Mechanical

dc.title

Curved FtsZ protofilaments generate bending forces on liposome membranes.

dc.type

Journal article

duke.contributor.orcid

Erickson, Harold P|0000-0002-9104-8987

pubs.begin-page

3476

pubs.end-page

3484

pubs.issue

22

pubs.organisational-group

School of Medicine

pubs.organisational-group

Duke Cancer Institute

pubs.organisational-group

Biochemistry

pubs.organisational-group

Cell Biology

pubs.organisational-group

Duke

pubs.organisational-group

Institutes and Centers

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Faculty

pubs.publication-status

Published

pubs.volume

28

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